Chicken anti-GFP (ab13970) antibody was purchased from Abcam. Rat anti-E-cadherin (131900) antibody was from Life Technologies. Alexa-Fluor-647-conjugated rabbit anti-cleaved caspase-3 (9602S) antibody was purchased from Cell Signaling Technology. Alexa-Fluor-568-conjugated phalloidin from Life Technologies was used at 1.0 U/mL. Alexa-488-conjugated secondary antibody was from Abcam, and Alexa-Fluor-568- and 647-conjugated secondary antibodies were from Life Technologies. Hoechst 33342 (Life Technologies) was used at a dilution of 1:5,000. TMRM was obtained from Molecular Probes. Palmitic acid, stearic acid, linoleic acid, α-linolenic acid, sphingomyelin, cholesterol, and Nile Red were from Wako Pure Chemicals Industries. Acetylsalicylic acid (aspirin) was from Sigma-Aldrich. Trimetazidine (Abcam) was used at 10 μM.
Stearic acid
Manufactured by Fujifilm
Sourced in Japan
Stearic acid is a long-chain fatty acid commonly used in laboratory equipment and scientific applications. It is a solid, white, odorless substance at room temperature. Stearic acid serves as an important component in various laboratory processes, providing specific physical and chemical properties essential for certain experiments and analyses.
Automatically generated - may contain errors
Lab products found in correlation
Tributyrin, by Fujifilm (4 mentions)
Myristic acid, by Fujifilm (3 mentions)
Butyl stearate, by Fujifilm (3 mentions)
Hoechst 33342, by Thermo Fisher Scientific (2 mentions)
Alexa fluor 568 conjugated phalloidin, by Thermo Fisher Scientific (2 mentions)
Acetylsalicylic acid aspirin, by Merck Group (2 mentions)
Alexa 488 conjugated secondary antibody, by Abcam (2 mentions)
Sphingomyelin, by Fujifilm (2 mentions)
Cholesterol, by Fujifilm (2 mentions)
Palmitic acid, by Fujifilm (2 mentions)
Linoleic acid, by Fujifilm (2 mentions)
Chicken anti gfp ab13970 antibody, by Abcam (2 mentions)
Rat anti e cadherin 131900 antibody, by Thermo Fisher Scientific (2 mentions)
Alexa fluor 647 conjugated rabbit anti cleaved caspase 3 9602s antibody, by Cell Signaling Technology (2 mentions)
α linolenic acid, by Fujifilm (2 mentions)
Trimetazidine, by Abcam (2 mentions)
Alexa fluor 568 and 647 conjugated secondary antibodies, by Thermo Fisher Scientific (2 mentions)
Nile red, by Fujifilm (2 mentions)
Linoleic acid, by Merck Group (1 mentions)
α tocopherol, by Fujifilm (1 mentions)
14 protocols using stearic acid
1
Immunolabeling of Apoptosis and Cytoskeleton
2
Immunolabeling of Apoptosis and Cytoskeleton
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Chicken anti-GFP (ab13970) antibody was purchased from Abcam. Rat anti-E-cadherin (131900) antibody was from Life Technologies. Alexa-Fluor-647-conjugated rabbit anti-cleaved caspase-3 (9602S) antibody was purchased from Cell Signaling Technology. Alexa-Fluor-568-conjugated phalloidin from Life Technologies was used at 1.0 U/mL. Alexa-488-conjugated secondary antibody was from Abcam, and Alexa-Fluor-568- and 647-conjugated secondary antibodies were from Life Technologies. Hoechst 33342 (Life Technologies) was used at a dilution of 1:5,000. TMRM was obtained from Molecular Probes. Palmitic acid, stearic acid, linoleic acid, α-linolenic acid, sphingomyelin, cholesterol, and Nile Red were from Wako Pure Chemicals Industries. Acetylsalicylic acid (aspirin) was from Sigma-Aldrich. Trimetazidine (Abcam) was used at 10 μM.
3
Fatty Acid and Antioxidant Dietary Effects
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Flies were reared on either a plain diet (Formula 4–24 Plain Drosophila Medium; Carolina Biological Supply); a diet with the addition of one of the following FAs at 5 μL/mL unless otherwise indicated: linoleic acid (L1012; Sigma-Aldrich), stearic acid (198-12481; Wako), or myristic acid (134-03435; Wako); or a diet with 25 IU/mL α-tocopherol (207-01792; Wako) as reported previously (24 (link), 77 (link), 78 (link)).
4
Silver Paste Composition Preparation
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Example 4
As the silver particles, AgC239 (FUKUDA METAL FOIL & POWDER Co., Ltd.) was used. As the solvent, tributyrin (boiling point 305° C., Wako Pure Chemical Industries, Ltd.), butyl stearate (boiling point 343° C., Wako Pure Chemical Industries, Ltd.), and stearic acid (boiling point 376° C., Wako Pure Chemical Industries, Ltd.) were used. The silver particles and the solvent were kneaded for 15 minutes with a stone mill at the blending ratio shown in Table 1 to manufacture a silver paste composition. The properties of this silver paste composition were measured to obtain the result shown in Table 1.
5
Synthesis and Characterization of 2-tDCB for Carcinogenesis Study
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2-tDCB (purity: >99.3%) was synthesized at Hayashi Pure Chemical Ind., Ltd., Osaka,
Japan (Fig. 1A ![]()
The concentration of 2-tDCB in the powder diet (n=3/group) was determined by gas
chromatography-mass spectrometry (GC-MS), and the stability of 2-tDCB after 72 h of
storage at room temperature was confirmed. AOM and stearic acid were purchased from Wako
Pure Chemical Industries, Ltd. (Osaka, Japan).
Japan (
Structure of 2-tDCB and experimental design for AOM-induced carcinogenesis. A:
2-ACBs have a cyclobutanone ring and an alkyl chain. The alkyl chain length differs
depending on the precursor fatty acid. B: For the two-stage carcinogenesis study,
AOM was dissolved in 0.9% NaCl and administered subcutaneously in 6-, 7- and
8-week-old rats. At 9 weeks of age, administration of the powder diet containing
2-tDCB was begun.
The concentration of 2-tDCB in the powder diet (n=3/group) was determined by gas
chromatography-mass spectrometry (GC-MS), and the stability of 2-tDCB after 72 h of
storage at room temperature was confirmed. AOM and stearic acid were purchased from Wako
Pure Chemical Industries, Ltd. (Osaka, Japan).
6
Fatty Acid and Lipid Standards
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Caprylic acid (C8:0), capric acid (C10:0), lauric acid (C12:0), myristic acid (C14:0), stearic acid (C18:0), arachidonic acid (C20:4n6), and methyl-d3 stearate standard were obtained from Wako Pure Chemicals (Tokyo, Japan).
7
Synthesis of Click-Functionalized Amphiphiles
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11-Azido-3,6,9-trioxaundecan-1-amine (>93.0%), 10-undecynoic acid (>98.0%), tetraethylene glycol monomethyl ether (>98.0%), and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (>98.0%) were purchased from TCI (Japan). Stearic acid (>95.0%), 4-dimethylaminopyridine (>99.0%), and CuCl (>95.0%) were procured from Wako Chemicals (Japan). N,N'-dicyclohexylcarbodiimide (>98.0%) was purchased from Kanto Chemical (Japan). All solvents and reagents were used as received. 1H- and 13C-NMR spectra were recorded on an AV-500 spectrometer (Bruker, USA) operating at 500 MHz and 125 MHz respectively. CDCl3 was used as the solvent. 1H-NMR spectra were referenced to tetramethylsilane (TMS) and 13C-NMR spectra were referenced to the deuterated solvent CDCl3. High resolution electrospray ionization mass spectrometry (HR-ESI-MS) was performed by using a JMS-T100LC spectrometer (JEOL, Japan).
8
Organic Acid Intercalation into LDH Nanohybrids
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The organic monocarboxylic acids sodium salts; n-capric acid (CH3(CH2)8COOH), myristic acid (CH3(CH2)12COOH), and stearic acid (CH3(CH2)16COOH) were obtained from WAKO Company (Tokyo, Japan). The supplies of dicarboxylic acids sodium salts (suberic acid COOH(CH2)6COOH and sebacic acid COOH(CH2)8COOH) were from TCI Company (Tokyo, Japan). The nanohybrids were formed through anions exchange reactions. Typically, the appropriate concentration of organic acid (0.2 M) was prepared through dissolving 0.002 mol of the sodium salt of aliphatic acids in 10 mL of deionized–distilled water. By using ultrasonic technique, this aqueous solution of organic acid was mixed with 0.5 g of the prepared Co/Zn/Ti LDH for performing intercalation reactions. This process was achieved under an argon atmosphere with strong stirring at room temperature for 24 h. The product was filtrated and washed many times by deionized–distilled water. The fine powder was obtained after drying under vacuum.
9
Synthesis of Cosmetic Formulations
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Taiyo Kagaku Co., Ltd. (Mie, Japan) gifted the l -theanine. Stearic acid and propylene glycol were purchased from FUJIFILM Wako Pure Chemical Corp. (Osaka, Japan). Stearyl alcohol was purchased from Nacalai Tesque, INC. (Kyoto, Japan). All other chemicals were commercial products of reagent grade.
10
Emulsion Solvent-Evaporation Synthesis of Magnetic Polystyrene Nanoparticles
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Materials PS (Mw 280000) was purchased from Sigma-Aldrich (St. Louis, MO, U.S.A.). Iron oxide NPs (diameter: 10 nm) were purchased from Ferrotec Material Technologies (Tokyo, Japan). Chloroform (CHCl 3 ), stearic acid (SA), and methanol (MeOH) were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). C-Reactive protein (CRP)-monoclonal antibody (mAb) and CRP-immunoglobulin G (IgG) were purchased from Oriental Yeast (Tokyo, Japan). Horseradish peroxidase (HRP)-conjugated AffiniPure goat anti-rabbit (aRb) IgG was purchased from Jackson Immu-noResearch (West Grove, PA, U.S.A.).
Preparation of MPS NPs by Emulsion Solvent-Evaporation MPS NPs were synthesized by emulsion solventevaporation in accordance with a previous report 21) (link) with major modifications. In brief, a CHCl 3 solution (450 µL) consisting of iron oxide NPs (4.23 mg), PS (1.02 mg), and SA (45 µg) was added to 4 mL of H 2 O or 4% (v/v) aqueous MeOH at room temperature. The mixture was treated with an ultrasonic homogenizer under an ice bath for several minutes followed by incubating at room temperature overnight to evaporate the CHCl 3 . Finally, the resulting MPS NPs (3 mL) were washed with water 3× under a magnet and re-dispersed in water (3 mL). The MPS NPs were stored at room temperature.
Preparation of MPS NPs by Emulsion Solvent-Evaporation MPS NPs were synthesized by emulsion solventevaporation in accordance with a previous report 21) (link) with major modifications. In brief, a CHCl 3 solution (450 µL) consisting of iron oxide NPs (4.23 mg), PS (1.02 mg), and SA (45 µg) was added to 4 mL of H 2 O or 4% (v/v) aqueous MeOH at room temperature. The mixture was treated with an ultrasonic homogenizer under an ice bath for several minutes followed by incubating at room temperature overnight to evaporate the CHCl 3 . Finally, the resulting MPS NPs (3 mL) were washed with water 3× under a magnet and re-dispersed in water (3 mL). The MPS NPs were stored at room temperature.
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