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3 3 diaminobenzidine tetrahydrochloride dab immunostaining detection reagent

Manufactured by Thermo Fisher Scientific
Sourced in United States, United Kingdom

3,3′-Diaminobenzidine tetrahydrochloride (DAB) is a chromogenic substrate used in immunohistochemistry and immunocytochemistry for the detection of target antigens. It produces a brown insoluble precipitate at the site of the antigen-antibody reaction, allowing for visualization of the target protein.

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2 protocols using 3 3 diaminobenzidine tetrahydrochloride dab immunostaining detection reagent

1

Immunohistochemical Analysis of Hypoxia Markers

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RPMI 1640 medium, fetal bovine serum (FBS), and antibiotics (penicillin/streptomycin) were purchased from HyClone (South Logan, UT, USA). Anti-HIF-1α, anti-LOX, anti-Oct3/4, and anti-Notch-4 antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-CD11b and anti-ALDH1 antibodies were purchased from Abcam (Cambridge, UK). Enhanced chemiluminescence (ECL) western blotting detection reagent was obtained from Bio-Rad (Hercules, CA, USA). 3,3′-Diaminobenzidine tetrahydrochloride (DAB) immunostaining detection reagent was obtained from Thermo Fisher Scientific (Waltham, MA, USA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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2

Antibody and Chemical Reagents for Biological Assays

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Anti-HIF-1α and anti-LOX antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-CD11b antibody was purchased from Abcam (Cambridge, UK). Enhanced chemiluminescence (ECL) Western blotting detection reagent was obtained from Amersham (Buckinghamshire, UK). 3,3%-Diaminobenzidine tetrahydrochloride (DAB) immunostaining detection reagent was obtained from Thermo (Runcorn, UK). All other chemicals, including ATP, UTP,γATP, apyrase, and β-aminopropionitrile (βAPN), were purchased from Sigma-Aldrich (St. Louis, MO).
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