3 3 diaminobenzidine tetrahydrochloride dab immunostaining detection reagent

Manufactured by Thermo Fisher Scientific

Sourced in United States, United Kingdom

3,3′-Diaminobenzidine tetrahydrochloride (DAB) is a chromogenic substrate used in immunohistochemistry and immunocytochemistry for the detection of target antigens. It produces a brown insoluble precipitate at the site of the antigen-antibody reaction, allowing for visualization of the target protein.

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Lab products found in correlation

Anti hif 1α, by Santa Cruz Biotechnology (1 mentions) Anti oct3 4, by Santa Cruz Biotechnology (1 mentions) Anti lox, by Santa Cruz Biotechnology (1 mentions) Anti notch4, by Santa Cruz Biotechnology (1 mentions) Enhanced chemiluminescence ecl western blotting detection reagent, by Bio-Rad (1 mentions) Anti cd11b antibody, by Abcam (1 mentions) Apyrase, by Merck Group (1 mentions) Enhanced chemiluminescence ecl western blotting detection reagent, by Cytiva (1 mentions) Adenosine triphosphate (atp), by Merck Group (1 mentions)

2 protocols using 3 3 diaminobenzidine tetrahydrochloride dab immunostaining detection reagent

Immunohistochemical Analysis of Hypoxia Markers

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RPMI 1640 medium, fetal bovine serum (FBS), and antibiotics (penicillin/streptomycin) were purchased from HyClone (South Logan, UT, USA). Anti-HIF-1α, anti-LOX, anti-Oct3/4, and anti-Notch-4 antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-CD11b and anti-ALDH1 antibodies were purchased from Abcam (Cambridge, UK). Enhanced chemiluminescence (ECL) western blotting detection reagent was obtained from Bio-Rad (Hercules, CA, USA). 3,3′-Diaminobenzidine tetrahydrochloride (DAB) immunostaining detection reagent was obtained from Thermo Fisher Scientific (Waltham, MA, USA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Ko Y.S., Rugira T., Jin H., Joo Y.N, & Kim H.J. (2020). Radiotherapy-Resistant Breast Cancer Cells Enhance Tumor Progression by Enhancing Premetastatic Niche Formation through the HIF-1α-LOX Axis. International Journal of Molecular Sciences, 21(21), 8027.

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Antibody and Chemical Reagents for Biological Assays

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Anti-HIF-1α and anti-LOX antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-CD11b antibody was purchased from Abcam (Cambridge, UK). Enhanced chemiluminescence (ECL) Western blotting detection reagent was obtained from Amersham (Buckinghamshire, UK). 3,3%-Diaminobenzidine tetrahydrochloride (DAB) immunostaining detection reagent was obtained from Thermo (Runcorn, UK). All other chemicals, including ATP, UTP,γATP, apyrase, and β-aminopropionitrile (βAPN), were purchased from Sigma-Aldrich (St. Louis, MO).

Joo Y.N., Jin H., Eun S.Y., Park S.W., Chang K.C, & Kim H.J. (2014). P2Y2R activation by nucleotides released from the highly metastatic breast cancer cell contributes to pre-metastatic niche formation by mediating lysyl oxidase secretion, collagen crosslinking, and monocyte recruitment. Oncotarget, 5(19), 9322-9334.

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