Uas sod1

Manufactured by Bloomington Drosophila Stock Center

The UAS-SOD1 is a genetic construct that allows for the overexpression of the superoxide dismutase 1 (SOD1) gene in Drosophila melanogaster. SOD1 is an enzyme that plays a crucial role in the defense against oxidative stress. This construct can be used to study the effects of SOD1 overexpression in various biological processes and disease models.

Automatically generated - may contain errors

Lab products found in correlation

8 protocols using uas sod1

Visualizing mitochondria and DCVs in motor neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols

w;+;D42-Gal4, UAS-mitoGFP (Dr. Pallanck, University of Washington) and w;sp/Cyo;D42-Gal4, UAS-ANF-GFP (Dr. Saxton, UC Santa Cruz) were used to visualize GFP signals in mitochondria and dense core vesicles (DCVs), respectively, in motor neurons. UAS-SOD1 and UAS-SOD2 from the Bloomington Stock Center were crossed with w;+;D42-Gal4, UAS-mitoGFP to generate UAS-SOD1; D42-Gal4, UAS-mitoGFP and UAS-SOD1; D42-Gal4, UAS-mitoGFP. We used these two strains to perform the rescue experiments in Figs 1, 2 and 4. To examine calcium (Ca²⁺) levels in motor neurons, we received fly strains UAS-GCaMP6m from the Bloomington Stock Center, and generated UAS-GCaMP6m/+; D42-Gal4/+. To activate or down-regulate the JNK pathway, we used UAS-Hep^B2 (Bloomington Stock Center) and UAS-Bsk RNAi (VDRC), respectively. These fly strains were also crossed with the control strain, w;+;D42-Gal4, UAS-mitoGFP, to generate UAS-Hep^B2; D42-Gal4, UAS-mitoGFP and UAS-Bsk RNAi; D42-Gal4, UAS-mitoGFP. All flies were maintained in normal fly food at 25°C with 40%-60% humidity and a 12 hr light/dark cycle.

Liao P.C., Tandarich L.C, & Hollenbeck P.J. (2017). ROS regulation of axonal mitochondrial transport is mediated by Ca2+ and JNK in Drosophila. PLoS ONE, 12(5), e0178105.

+ Open protocol

+ Expand

Drosophila Genetic Strains for Research

Cited 3 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

w¹¹¹⁸ or genetic background strains were used as control lines in this study. Fly strains used include: Canton-S (Bloomington Drosophila Stock Center, Stock 64349), w¹¹¹⁸, SK^-/- (kindly provided by Dr. Patrick Dolph) [44 (link)], Df(Shaker) (kindly provided by Dr. Kyunghee Koh) [45 (link)], UAS-DNK_v4 [40 (link)], UAS-K_v4/Shal [43 (link)], UAS-K_v1/Shaker (kindly provided by Dr. William Joiner), and UAS-SOD1, UAS-SOD2, UAS-Catalase, UAS-NOX-RNAi and UAS-DUOX-RNAi (all kindly provided by Dr. Matthias Landgraf), UAS-GFP-K_v4/Shal [46 (link)], and elav-GAL4, tub-GAL80^ts, UAS-Dcr² (all obtained from the Bloomington Drosophila Stock Center).

Vallejos M.J., Eadaim A., Hahm E.T, & Tsunoda S. (2021). Age-related changes in Kv4/Shal and Kv1/Shaker expression in Drosophila and a role for reactive oxygen species. PLoS ONE, 16(12), e0261087.

+ Open protocol

+ Expand

Drosophila Mitochondrial Genetics Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

w1118 (#6326), UAS-mitoGFP (#8442), UAS-SOD1 (#33605 and #24750), UAS-SOD2 (#24494) were obtained from the Bloomington Stock Center. UAS-MARF RNAi, UAS-Opa1 RNAi, and UAS-Drp1 TG were provided by M. Guo (University of California, Los Angeles, CA) [15 (link)]. UAS-Romo1 RNAi (#v101353 and #v9224) were obtained from the Vienna Drosophila RNAi Center [16 ]. The strain expressing XBP1 (XBP1 ^d08698) was obtained from Exelixis collection at Harvard. UAS transgenes were expressed in Drosophila cardiomyocytes using the tincΔ4-Gal4 driver provided by Rolf Bodmer (Sanford-Burnham Medical Research Institute, La Jolla, CA) [17 (link)].

Bhandari P., Song M, & Dorn GW I.I. (2014). Dissociation of mitochondrial from sarcoplasmic reticular stress in Drosophila cardiomyopathy induced by molecularly distinct mitochondrial fusion defects. Journal of molecular and cellular cardiology, 80, 71-80.

+ Open protocol

+ Expand

Genetic Manipulation of Scribble-Ras Oncogenic Axis

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The scrib allele used is scrib² (also known as scrib⁶⁷³) (Bilder and Perrimon, 2000 (link)). The recombinant UAS-Ras^V12 FRT82B scrib² (Chen et al., 2012 (link); Pérez et al., 2015 (link)) line was a kind gift of Madhuri Kango-Singh (U Dayton, OH, USA). The MARCM system (Lee and Luo, 1999 (link)) with ey-FLP (Newsome et al., 2000 (link)) was used to generate mosaics of eye/antennal imaginal discs and experimental clones were marked by GFP. In (Figure 1—figure supplement 1), we used a modified MARCM system that marks clones with myrRFP (Chabu and Xu, 2014 (link)). The wt control line (FRT +) is FRT82B (Xu and Rubin, 1993 (link)).
The following transgenes are all inserted on chromosome 2 and were crossed into the scrib^−/−Ras^V12 background for analysis: UAS-lacZ (Bloomington, BL3955), UAS-Duox RNAi and UAS-hCatS (a kind gift of Won-Jae Lee) (Ha et al., 2005a (link); Ha et al., 2005b2005 (link)), UAS-Catalase (BL24621), UAS-SOD1 (BL24754), UAS-SOD2 (BL24494), UAS-p35 (BL5072), UAS-dronc RNAi and UAS-drICE RNAi (a kind gift of Pascal Meier) (Leulier et al., 2006 (link)). UAS-JNK^DN (aka UAS-bsk^DN) (BL6409) is an insertion on X chromosome. Crosses were incubated at either 22° or 25°C.

Pérez E., Lindblad J.L, & Bergmann A. (2017). Tumor-promoting function of apoptotic caspases by an amplification loop involving ROS, macrophages and JNK in Drosophila. eLife, 6, e26747.

+ Open protocol

+ Expand

Transgenic Fly Line Generation

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flies were maintained in standard cornmeal media at 25°C under light–dark cycles of 12:12 hours. To establish the transgenic fly line carrying the human 2N4R tau with or without mutations or deletions, cDNA was subcloned into pUASTattB, and injected to the oocytes carrying into P{CaryP}attP2. UAS-SOD1 (31 (link)) was obtained from Bloomington stock center (Stock #33605). For RU486 feeding, RU486 was mixed into the cornmeal media at the final concentration of 500 μm. Genotypes of the flies used in the experiments are described in Supplementary Material Table S1.

Saito T., Chiku T., Oka M., Wada-Kakuda S., Nobuhara M., Oba T., Shinno K., Abe S., Asada A., Sumioka A., Takashima A., Miyasaka T, & Ando K. (2021). Disulfide bond formation in microtubule-associated tau protein promotes tau accumulation and toxicity in vivo. Human Molecular Genetics, 30(21), 1955-1967.

+ Open protocol

+ Expand

Drosophila Genetics for Cell Stress Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following strains were obtained from repositories: UAS-Sod1 (RRID:BDSC_24754), UAS-Duox^RNAi (RRID:BDSC_32903, RRID:BDSC_33975), UAS-ATRIP^RNAi (RRID:BDSC_61355), UAS-TOPBP1^RNAi (RRID:BDSC_43244), UAS-Claspin^RNAi (RRID:BDSC_32974) (Bloomington Drosophila Stock Center), UAS-Chk1^RNAi (RRID:FlyBase_FBst0473748), and UAS-ATR^RNAi (RRID:FlyBase_FBst0475838; Vienna Drosophila Resource Center). UAS-Chk1 was generated in the in-house fly facility. The following strains were received as gifts: btl-GAL4, UAS-ATR, and RPA70-GFP. Strains were raised on a diet of cornmeal-agar and maintained at 25°C. All experiments were performed on animals raised at 25°C unless otherwise indicated.

Kizhedathu A., Chhajed P., Yeramala L., Sain Basu D., Mukherjee T., Vinothkumar K.R, & Guha A. (2021). Duox-generated reactive oxygen species activate ATR/Chk1 to induce G2 arrest in Drosophila tracheoblasts. eLife, 10, e68636.

+ Open protocol

+ Expand

Drosophila Genetic Manipulation Toolkit

Check if the same lab product or an alternative is used in the 5 most similar protocols

UAS-catalase, UAS-SOD1, UAS-SOD2, UAS-p38b⁺, UAS-DMKK3⁺, UAS-coracle⁺, Tubulin-GAL4, Armadillo-GAL4 (Arm-GAL4), and Tubulin-Gal80^ts were from the Bloomington Stock Center. UAS-catalase^RNAi, UAS-SOD1^RNAi, UAS-SOD2^RNAi, UAS-DMKK3^RNAi, and UAS-coracle^RNAi were from the Vienna Drosophila RNAi Center. D-p38a¹³ and D-p38b^156A were as previously described (Chen et al., 2010 (link)). UAS-p38b^DN (Adachi-Yamada et al., 1999 (link)) was a kind gift from T. Adachi-Yamada at Kobe University, Japan. Dot-GAL4 (insertion 11C (c2)) (Kimbrell et al., 2002 (link)) was previously generated in D.A. Kimbrell’s laboratory at the University of California, Davis. GMH5 was as previously described by Wessells et al. (2004) (link). Hand-GAL4 was a kind gift from A. Paululat (University of Osnabruek, Germany).

Lim H.Y., Wang W., Chen J., Ocorr K, & Bodmer R. (2014). ROS Regulate Cardiac Function via a Distinct Paracrine Mechanism. Cell reports, 7(1), 35-44.

+ Open protocol

+ Expand

Drosophila Genetic Manipulation Toolkit

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lim H.Y., Wang W., Chen J., Ocorr K, & Bodmer R. (2014). ROS Regulate Cardiac Function via a Distinct Paracrine Mechanism. Cell reports, 7(1), 35-44.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!