Solarix xr 7t

Polar metabolites and lipids were extracted as reported previously [31 (link)]. HRMS analyses were performed in flow injection analysis (FIA) by using an Ultimate 3000 UHPLC (Thermo, Bremen, Germany) coupled to a SolariX XR 7T (Bruker Daltonics, Bremen, Germany). The flow rate was set to 10 mL/min and increased in the washing step to 300 mL/min. The instrument was tuned with a standard solution of sodium trifluoracetate (NaTFA). Mass spectra were recorded in a broadband mode in the range of 150–1500 m/z for lipids, whereas 90–800 m/z was used for polar metabolites, with an ion accumulation of 10 ms. A total of 64 scans were acquired using 4 million data points (4 M), with an approximate resolution of 400.000 at m/z 400. Drying gas (nitrogen) was set at 2 mL/min, with a drying temperature of 150 °C. Funnel amplitude was set to 90 V (polar metabolites) or 100 V (lipids), transfer was set at 0.6 MHz, and TOF 0.7 s. Both positive and negative ESI ionization was employed in a separate run. Five replicates of each injection were carried out. The instrument was controlled by Bruker FTMS Control (Bruker). FIA-FT-ICR data extraction, alignment, filtering, and annotation were performed with Metaboscape (v. 5.0, Bruker), as reported previously [31 (link)].

Analyses were performed in direct infusion employing a Hamilton syringe (250 μL) at a flow rate of 2 μL/min. Data were acquired on a SolariX XR 7 T (Bruker Daltonics, Bremen, Germany). The instrument was tuned with a standard solution of sodium trifluoracetate (NaTFA). Mass spectra were recorded in broadband mode in the range 100–1500 m/z, with an ion accumulation of 20 ms, with 32 scans using 2 million data points (2 M). Nebulising (nitrogen) and drying (air) gases were set at 1.0 and 4.0 mL/min, respectively, with a drying temperature of 200 °C. The instrument was controlled by Bruker FTMS Control, MS spectra were elaborated with Compass Data Analysis version 4.2 (Bruker Daltonics, Bremen, Germany) and identification of compounds based on accurate MS measurements was performed by Compound Crawler ver. 3.0 (Bruker).