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Sipkm2

Manufactured by Santa Cruz Biotechnology
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Sourced in United States

SiPKM2 is a laboratory equipment product manufactured by Santa Cruz Biotechnology. It is a protein kinase module designed for use in scientific research. The core function of SiPKM2 is to facilitate the study of protein kinase-mediated signaling pathways.

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Lab products found in correlation

P erk, by Cell Signaling Technology (1 mentions) P egfr, by Cell Signaling Technology (1 mentions) Sodium arsenite naaso2, by Merck Group (1 mentions) Crm197, by Merck Group (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Hbegf, by Santa Cruz Biotechnology (1 mentions) Si ampkα1, by RiboBio (1 mentions)

2 protocols using sipkm2

1

Exploring BEAS-2B and As-T Cell Cultures

Cited 1 time
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Human bronchial epithelial BEAS-2B (BEAS-2B) and As-T cells were established and cultured in DMEM medium mixed with 10% heat-inactivated fetal bovine serum (FBS), 100 units/mL penicillin G, and 100 μg/mL streptomycin, as previously described (21 (link)). Sodium arsenite (NaAsO2) and CRM197 were purchased from Sigma Aldrich (St. Louis, MO). EGFR, p-EGFR, PKM2, p-ERK, β-actin antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). SiHB-EGF, HB-EGF, PKM2 and siPKM2 were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).
Wang L., Lu Y.F., Wang C.S., Xie Y.X., Zhao Y.Q., Qian Y.C., Liu W.T., Wang M, & Jiang B.H. (2020). HB-EGF Activates the EGFR/HIF-1α Pathway to Induce Proliferation of Arsenic-Transformed Cells and Tumor Growth. Frontiers in Oncology, 10, 1019.
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2

Cellular Metabolism Modulation Protocols

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SiPKM2 was bought from Santa Cruz Biotechnology, siAMPKα1 and corresponding negative control (NC) were purchased from Guangzhou Ribobio Co., PKM2 overexpressed plasmid and corresponding negative control were purchased from Shanghai GeneChem Co., SiRNAs were transfected with Lipofectamine 2000 at a final concentration of 50 nM, while plasmids were transfected with 0.2 μg for 96-well plate and 1.6 μg for 12-well plate. The effects of the transient transfection were supported by qRT-PCR and western blot analysis.
Li X., Deng S., Liu M., Jin Y., Zhu S., Deng S., Chen J., He C., Qin Q., Wang C, & Zhao G. (2018). The responsively decreased PKM2 facilitates the survival of pancreatic cancer cells in hypoglucose. Cell Death & Disease, 9(2), 133.
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