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Aqua mount media

Manufactured by Polysciences
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Sourced in United Kingdom

Aqua-mount media is an aqueous-based mounting medium designed for use in microscopy. It is formulated to preserve the optical properties of specimens while providing a clear, non-drying mounting solution.

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Lab products found in correlation

Oregon green 488 conjugated gelatin, by Thermo Fisher Scientific (2 mentions) Hoechst, by Thermo Fisher Scientific (2 mentions) Rhodamine conjugated phalloidin, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Aqua-Poly/Mount Media Aqua-Poly/Mount mounting media

2 protocols using aqua mount media

1

Visualizing Melanoma Cell Actin Cytoskeleton

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Coverslips were coated with warm Oregon Green® 488-conjugated gelatin (Invitrogen) as described in Ref(9 (link)). Melanoma cells (7.5 × 104) were seeded on the coverslips and after 16-hour incubation at 37°C they were fixed in 4% paraformaldehyde in PBS. After permeabilization in 0.05% triton x-100 in PBS, cells were stained with rhodamine-conjugated phalloidin (Invitrogen, to visualize actin) and hoechst (Thermo Scientific, to visualize nuclei). Coverslips were mounted onto glass slides with aqua-mount media (Polysciences, Warrington, PA). Fluorescent images were captured using a Nikon TE2000-E inverted microscope equipped with Roper CoolSnap HQ CCD camera and MetaVue software.
Bianchi-Smiraglia A., Bagati A., Fink E.E., Moparthy S., Wawrzyniak J.A., Marvin E.K., Battaglia S., Jowdy P., Kolesnikova M., Foley C.E., Berman A.E., Kozlova N.I., Lipchick B.C., Paul-Rosner L.M., Bshara W., Ackroy J., Shewach D.S, & Nikiforov M.A. (2016). Microphthalmia-Associated Transcription Factor Suppresses Invasion by Reducing Intracellular GTP Pools. Oncogene, 36(1), 84-96.
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2

Visualizing Melanoma Cell Actin Cytoskeleton

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Coverslips were coated with warm Oregon Green® 488-conjugated gelatin (Invitrogen) as described in Ref(9 (link)). Melanoma cells (7.5 × 104) were seeded on the coverslips and after 16-hour incubation at 37°C they were fixed in 4% paraformaldehyde in PBS. After permeabilization in 0.05% triton x-100 in PBS, cells were stained with rhodamine-conjugated phalloidin (Invitrogen, to visualize actin) and hoechst (Thermo Scientific, to visualize nuclei). Coverslips were mounted onto glass slides with aqua-mount media (Polysciences, Warrington, PA). Fluorescent images were captured using a Nikon TE2000-E inverted microscope equipped with Roper CoolSnap HQ CCD camera and MetaVue software.
Bianchi-Smiraglia A., Bagati A., Fink E.E., Moparthy S., Wawrzyniak J.A., Marvin E.K., Battaglia S., Jowdy P., Kolesnikova M., Foley C.E., Berman A.E., Kozlova N.I., Lipchick B.C., Paul-Rosner L.M., Bshara W., Ackroy J., Shewach D.S, & Nikiforov M.A. (2016). Microphthalmia-Associated Transcription Factor Suppresses Invasion by Reducing Intracellular GTP Pools. Oncogene, 36(1), 84-96.
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