Fastprep 24 grinder machine

Mycelial tissues of the 30 isolated fungi (Table 1) previously grown on PDA were ground by means the FastPrep^®-24 grinder machine (MP Biomedicals, Santa Ana, CA, USA). Subsequently, genomic DNA was extracted using the E.Z.N.A^® Fungal DNA Kit (OMEGA BioTek, Norcross, GA, USA). A MaestroNano^® spectrophotometer (MaestroGen, Taiwan) was used to determine the concentration and purity of the extracted DNA.

Genomic DNA was obtained from the 17 fungal isolates and six bacterial isolates included in this study. All of them were actively grown on potato dextrose agar (PDA, Difco^® Laboratories, Le Pont de Claix, France) at 27 ± 0.5 °C in the dark for 7 days. Mycelial tissues and bacterial cells were ground using a FastPrep^®-24 grinder machine (MP Biomedicals, Santa Ana, CA, USA). Subsequently, DNA extractions were performed using an E.Z.N.A.^® Fungal DNA Kit (OMEGA BioTek, Norcross, GA, USA) following the manufacturer’s instructions. A MaestroNano^® spectrophotometer (MaestroGen, Hsinchu City, Taiwan) was used to determine the concentration and purity of the extracted DNA.