Primescript rt master mix

Gene expression related to angiogenic growth factors was evaluated by qRT-PCR. hADSCs (1 × 10⁵) were seeded into PADM, FADM, LADM and FLADM of a well size of a 24-well plate and cultured for 48 h. Total RNA was extracted and reverse transcribed into cDNA with PrimeScript RT Master Mix using RNA purification kit and Reverse Transcription kit (with DNase) (EZBioscience). Gene expression levels of vascular endothelial growth factor (VEGF), insulin-like growth factor 1 (IGF-1) and epidermal growth factor (EGF) [24 (link)] were normalized to that of GAPDH and quantified with the comparative Ct method. The primers synthesized by Sangon Biotech Co. (Shanghai, China) are mentioned in Table 2.

HUVECs (1 × 10⁵) were seeded on circular PADM and FADM and cultured in a complete low glucose medium for 48 h. After cells were detached from the materials by trypsin (Gibco), total RNA was extracted and reverse transcribed into cDNA with PrimeScript RT Master Mix using RNA purification kit and Reverse Transcription kit (with DNase) (EZBioscience, USA). Gene expression levels of endothelial markers of CD105 (Endoglin/ENG), and Tie-2 (TEK receptor tyrosine kinase/TEK) [23 (link)] were normalized to that of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and quantified with the comparative Ct method. The primers synthesized by Sangon Biotech Co. (Shanghai, China) are mentioned in Table 2.

Gene expression related to angiogenic growth factors was evaluated by quantitative real-time PCR (RT-PCR). hADSCs (2×10⁵) were seeded into VT-ADM and GA-ADM in a 24-well plate and cultured for 48 h. Total RNA was extracted and reverse transcribed into cDNA with PrimeScript RT Master Mix using RNA purification kit and Reverse Transcription kit (with DNase) (EZBioscience, United States). Gene expression levels of vascular endothelial growth factor (VEGF), insulin-like growth factor 1 (IGF-1), and epidermal growth factor (EGF) were normalized to that of glyceraldehyde 3-phosphate dehydrogenase (GADPH) and quantified with the comparative Ct method. The primers synthesized by Sangon Biotech Co. (Shanghai, China) are mentioned in Table 1.