Ezh2 or h3k27me3 specific antibodies

Manufactured by Merck Group

EZH2 or H3K27me3-specific antibodies are laboratory reagents used in various research applications. These antibodies specifically recognize and bind to the EZH2 protein or the trimethylated form of histone H3 at lysine 27 (H3K27me3), which are important epigenetic markers. The core function of these antibodies is to facilitate the detection and analysis of the target proteins or modifications in research samples.

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Lab products found in correlation

Ez chip kit, by Merck Group (1 mentions)

Close spelling variants of this product

H3K27me3‐specific antibodies Specific antibodies H3K27me3

3 protocols using ezh2 or h3k27me3 specific antibodies

Chromatin Immunoprecipitation for Epigenetic Profiling

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Chromatin immunoprecipitation (ChIP) assay was conducted using the EZ-ChIP kit (Millipore). Cells were treated with formaldehyde and incubated for 10 min to generate DNA-protein crosslinks. Then the cell lysates were sonicated to generate chromatin fragments of 200–300 bp and immunoprecipitated with EZH2 or H3K27me3-specific antibodies (Millipore) or IgG as control. The precipitated chromatin DNA were analyzed by qRT-PCR assays. The information of the sequences of ChIP primers are listed in Additional file 1: Table S3.

Zang X., Gu J., Zhang J., Shi H., Hou S., Xu X., Chen Y., Zhang Y., Mao F., Qian H., Zhu T., Xu W, & Zhang X. (2020). Exosome-transmitted lncRNA UFC1 promotes non-small-cell lung cancer progression by EZH2-mediated epigenetic silencing of PTEN expression. Cell Death & Disease, 11(4), 215.

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Chromatin Immunoprecipitation of EZH2 and H3K27me3

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A549 and SPC-A1 cells were treated with formaldehyde and incubated for 10 min to generate DNA–protein crosslinks. Cell lysates were then sonicated to generate chromatin fragments of 200–300 bp and immunoprecipitated with EZH2 or H3K27me3-specific antibodies (Millipore) or IgG as control. Precipitated chromatin DNA was recovered and analyzed by qRT-PCR.

Chen Z., Chen X., Chen P., Yu S., Nie F., Lu B., Zhang T., Zhou Y., Chen Q., Wei C., Wang W, & Wang Z. (2017). Long non-coding RNA SNHG20 promotes non-small cell lung cancer cell proliferation and migration by epigenetically silencing of P21 expression. Cell Death & Disease, 8(10), e3092-.

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Chromatin Immunoprecipitation for EZH2 and H3K27me3

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PC-9/GR cells were treated with formaldehyde and incubated for 10 min to generate DNA–protein crosslinks. Cell lysates were then sonicated to generate chromatin fragments of 200–300 bp and immunoprecipitated with EZH2- or H3K27me3-specific antibodies (Millipore) or IgG as a control. Precipitated chromatin DNA was recovered and analyzed by qRT-PCR.

Xu T., Yan S., Wang M., Jiang L., Ma P., Lu B., Chen Q., Wei C, & Wang Z. (2020). LncRNA UCA1 Induces Acquired Resistance to Gefitinib by Epigenetically Silencing CDKN1A Expression in Non-small-Cell Lung Cancer. Frontiers in Oncology, 10, 656.

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