Dm2700m

Part microstructure in the XY and ZX orientation was determined by taking optical microscopy of parts printed on a Mark Two printer. Samples were cross-sectioned and mounted in SamplKwik Fast Cure Acrylic potting media (Buehler, Lake Bluff, IL, USA) followed by polishing on an E-Prep 4 Grinding/Polishing System (Allied High Tech Products, Compton, CA, USA). The polishing process included a brief two-minute abrasion at 15 N and 600 RPM head/platen speed with each of 180, 320, 600, and 1200 grit sandpaper. The final polish was achieved with MasterMet Colloidal Silica Polishing Suspension (Buehler, Lake Bluff, IL, USA) for 9 min at 12 N. Samples were imaged with an upright materials microscope (DM2700 M, Leica Microsystems, Wetzlar, Germany) fitted with an HD digital camera (MC170 HD, Leica Microsystems, Wetzlar, Germany) at 50X and 100X magnification.

After sacrificing,
we cut 0.5 cm of colon samples from the anus, fixed them in formalin,
and then embedded them in paraffin blocks. Next, 4 μm tissue
sections were cut and stained with hematoxylin and eosin (H&E)
for histological analysis under a light microscope (DM2700 M, Leica
Microsystems, Wetzlar, Germany). Tissues were scored for inflammation,
edema, goblet cell depletion, and epithelial damage in accordance
with the following criteria: 0 = none present, 1 = minimal change,
2 = mild change, 3 = moderate change, and 4 = severe change. The scores
for inflammation, edema, goblet cell depletion, and epithelial damage
were summed to obtain sum colon scores. The analysis was conducted
by trained professionals. Total scores for colitis (the total colitis
index) were then added, resulting in a combined histologic score ranging
from 0 to 16.^{18 (link)}

Video recordings of evaporating droplets and images of the resulting deposits were obtained by using a microscope (Omano Microscope OM2300S-V14, The Microscope Store, Roanoke, VA, USA) equipped with a recording camera (Summit Camera SK2-10X, The Microscope Store, Roanoke, VA, USA). The colored-film quality of all samples was observed using a high magnification microscope (DM2700M, Leica, Wetzlar, Germany) equipped with a camera (MC-190, Leica, Wetzlar, Germany). To quantify the difference in appearance of color banding between the resulting deposition of the electric field-treated and untreated samples, images of the concentric color bands were analyzed. One-dimensional radial color (RGB) profiles were measured from the center of each deposit to the edge with the ImageJ plugin RGBProfiler. The raw RGB pixel intensity data was smoothed in MATLAB with a running average filter (smooth, span of 63). The derivative of pixel intensity with respect to the normalized radius was calculated in Python (numpy.gradient, version 3.7, Python Software Foundation, DE, USA).

Paraffin-embedded sections of the liver and kidney tissues were de-paraffinized and rehydrated using graded alcohol concentration [25 (link)]. Antigen retrieval was achieved by boiling in 10 mmol/L sodium citrate buffer for 10 min and then steadily cooling to room temperature. Subsequently, the sections were blocked using 3% H₂O₂ in methanol for 15 min to inhibit endogenous peroxidase activity. Following washing in phosphate buffer saline (PBS), the sections were incubated overnight at 4 °C with monoclonal rabbit anti-p53 and anti-CD43 primary antibodies (Elabscience, Wuhan, China) at a dilution of 1:40. The sections were incubated with peroxidase-conjugated goat anti-rabbit immunoglobulin G (IgG) at the same dilution of 1:500 for 2 h at 37 °C. The sections were washed in PBS, developed in prepared DAB chromogen solution, lightly counterstained with hematoxylin, dehydrated, mounted, and visualized under the light microscope (Leica DM2700 M, Wetzlar, Germany). The degree of positivity of the proteins (CD43 and p53) in the hepatic and renal cytoplasm was quantified using Image J software [26 (link)]. The photomicrograph is a representative slide from five animals, from which 3 slides were quantified.

Materials: 4-aminopyridine, K₂CO₃, acetone and other chemical reagents were obtained from Sigma-Aldrich, Saint Louis, MO, USA. CdSe/ZnS QDs were purchased from Wuhan Jiayuan Quantum Dot Co., Ltd. Wuhan, China. The maximum emission wavelength is 625 nm ± 5 nm, and the size is 5–10 nm. The specification is 30 mg powder QDs dispersed in 10 mL n-hexane solvent.
Characterizations: ¹H NMR spectra were executed on a Bruker Avance III 400. Differential scanning calorimetry (DSC) examination was performed on a Perkin-Elmer DSC 8000 with a heating and cooling rate of 10 °C/min. The morphologies of fibers were observed using scanning electron microscopy (SEM, Zeiss EVO18, Oberkochen, BW, Germany), polarizing optical microscope (POM, LEICA DM2700 M, Wetzlar, Hesse, Germany) and transmission electron microscopy (TEM, Tecnai G2 F20, Hillsboro, OR, USA). The powder X-ray diffraction analysis (XRD) was implemented on a Philips X pert pro. The FT-IR analysis and the UV-vis analysis were measured using Nicolet 510P IR spectra and a UV/VIS/NIR spectrometer (Perkin-Elmer lambda 950, Waltham, MA, USA). Laser scanning confocal microscopy was recorded on the Zeiss LSM800.
Simulation method: All calculations were performed using the Gaussian 16 program. Geometry optimization calculations were performed using the B3LYP DFT-D3 method.