The largest database of trusted experimental protocols

3 protocols using ptbc1d1 ser237

1

AMPK Signaling Pathway Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Unless otherwise stated all reagents were from Sigma–Aldrich. Primary antibodies against pan-AMPK-α (#2532), AMPK-β1/2 (#4150), pAMPK Thr172 (#2531), AKT (#4685), ACC (#3876), pACC Ser79 (#3661), IRAP (#3808), rabbit FLAG (#2368), Raptor (#2280), pRaptor Ser792 (#2083) and TBC1D1 (#5929) were from Cell Signaling Technology. Other antibodies were pan-14-3-3 (sc-629) and GST (sc-138) from Santa Cruz Biotechnology Inc., GFP (118144600001; Roche), AMPK-γ1 (ab32508; Abcam), pTBC1D1 Ser237 (#07-2268; Merck-Millipore), β-actin (A1978) and mouse FLAG (F1804). Isoform-specific AMPK-α antibodies were described previously [34 (link)]. A769662 (Abcam) and AICAR (Tocris) were used.
+ Open protocol
+ Expand
2

Antibody-Based Analysis of AMPK Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies against AMPKα1/2, AMPKα2, p‐AMPKα1/2(Thr183/Thr172), phospho‐ACC (Ser79), ACC, and 14‐3‐3 were obtained from Abcam (San Francisco, CA, USA). TBC1D1 and p‐TBC1D1 (Ser237) were purchased from Merck Millipore (Darmstadt, Germany). Antibodies against HDAC5 and p38 MAPK were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against β‐actin were purchased from Sigma‐Aldrich (St. Louis, MO, USA). Antibodies against phospho‐p38 (Thr180/Tyr182), α‐tubulin, GLUT4, and lamin B were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Phospho‐HDAC5 (Thr498) antibodies were from Thermo Fisher Scientific (Rockford, IL, USA). Horseradish peroxidase (HRP)‐conjugated goat anti‐rabbit IgG and goat anti‐mouse secondary antibodies were purchased from Enzo Life Sciences (Farmingdale, NY, USA). Metrnl was obtained from Cusabio (Wuhan, Hubei, China), and 1, 2‐bis (o‐aminophenoxy) ethane‐N, N, N′, N′‐tetraacetic acid (BAPTA)‐AM was purchased from Abcam. Compound C and STO‐609 were obtained from Calbiochem (San Diego, CA, USA). Protein A‐agarose beads were obtained from GE Healthcare (Piscataway, NJ, USA). The fluorescent Ca2+ indicator Fluo‐3 AM and Hoechst 33342 were obtained from Invitrogen (Leiden, the Netherlands).
+ Open protocol
+ Expand
3

AMPK Signaling Pathway Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Unless otherwise stated all reagents were from Sigma-Aldrich. Primary antibodies against pan-AMPK-α (#2532), pAMPK Thr172 (#2531), ACC (#3876), pACC Ser79 (#3661), EGFR (#2232), IRAP (#3808), N-cadherin (#14215), Rab10 (#8127S) and rabbit FLAG (#2368) were from Cell Signaling Technology. CD36 (ab124515), Calmodulin (ab45689), GLUT1 (ab115730) and SERCA1 (ab129104) were from Abcam. From Santa Cruz Biotechnology Inc were antibodies against pan-14-3-3 (sc-629) and transferrin receptor (sc-65882). Other antibodies were EHBP1L1 (#PAB15793; Abnova), GFP (118144600001; Roche), HA.11 16B12 (901503; Biolegend), MICAL1 (14818–1-AP; proteintech), pTBC1D1 Ser237 (#07-2268; Merck-Millipore), VPS13C (HPA043356; Atlas Antibody), β actin (A1978), Tubulin (T9026) and mouse FLAG (F1804). VPS13A antibody was produced as previously described84 (link). Secondary antibodies used were conjugated with an AlexaFluor (Invitrogen) or HRP (Cell Signaling Technology or Jackson ImmunoResearch). AICAR was from Tocris.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!