The glucose was estimated by glucose estimation kit (K606-100) provided by Bio Vision Company and quantified (g. L-1) from a glucose standard curve.
K606 100
Manufactured by Abcam
Sourced in United States
K606-100 is a laboratory equipment product. It is a 100-unit package of the item.
Automatically generated - may contain errors
Lab products found in correlation
Glucose colorimetric assay kit, by Abcam (1 mentions)
K607 100, by Abcam (1 mentions)
Lactate colorimetric assay kit, by Abcam (1 mentions)
K627 100, by Abcam (1 mentions)
Enliten atp assay system, by Promega (1 mentions)
Lactate assay kit, by Abcam (1 mentions)
Ezrmi 13k, by Merck Group (1 mentions)
9 protocols using k606 100
1
Glucose Quantification via BioVision Kit
2
Glucose Measurement in OVCA Cells
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OVCA cells transfected with the indicated siRNAs or plasmids were cultured in DMEM for 36 h and then incubated with DMEM without L-glucose and phenol red for 8 h. The amount of glucose in these media was measured with a Glucose Colorimetric Assay kit according to the manufacturer's instructions (K606-100, BioVision, USA). Fresh DMEM was used for the negative control. The experiments were repeated three times.
3
DPT's Impact on Cell Metabolism
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To determine whether DPT treatment altered glucose utilization or lactate production in A549 and SK-MES-1 cells, cells were plated at 2×105 cells per flask and incubated at 37°C with 5% CO2. After allowing the cells to attach and grow for 24 hr, 16 nM DPT was added to the flasks and incubated at 37°C for 48 hr. The medium was collected by centrifugation to remove the cells, and glucose and lactate levels were detected by standard colorimetric assay kits for glucose (K606-100, BioVision, Milpitas, CA) and lactate (K607-100, BioVision) per the manufacturer’s instructions. Glucose consumption and lactate production was calculated as described in the previous report (22 (link)).
4
Glucose Colorimetric Assay Protocol
5
Cellular Metabolic Assays
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Cellular glutathione (GSH), glutathione peroxidase (GPx) and SOD were measured by ELISA according to the manufacturer’s instructions [35 (link)]. Cellular lactate production was measured using a lactate assay kit (#K607-100; BioVision, Milpitas, CA, USA) as described in a previous study. The glucose uptake rate was detected using a glucose absorption assay kit (#K606-100; BioVision) [36 (link)].
6
Metabolic Profiling of Hepatocellular Carcinoma
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Hepatocellular carcinoma cells are handled in accordance with the needs of the experiment. Using a Lactate Colorimetric Assay kit II, the buildup of lactate in the medium was found (K627-100, BioVision, USA). Utilizing a glucose colorimetric assay kit, the quantity of glucose was determined (K606-100, Bio Vision, USA). The ENLITEN® ATP Assay System (Promega) was used to quantify ATP release in order to examine the ATP in supernatants.
7
Cellular Antioxidant and Metabolic Assays
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Cellular glutathione (GSH), glutathione peroxidase (GPx) and SOD were measured via ELISA assay according to the manufacturer’s instructions. Cellular lactate production in the medium was measured via a lactate assay kit (#K607-100; BioVision, Milpitas, CA, USA) according to a previous study. The cancer glucose uptake rate was detected via a glucose absorption assay kit (#K606-100; BioVision) [46 (link)].
8
Metabolic Biomarkers Assessment Protocol
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Fasting serum was assayed for glucose by spectrophotometry (K606-100; BioVision Inc., Milpitas, CA, USA) and for insulin by ELISA (EZRMI-13K; MilliporeSigma, Burlington, MA, USA). Homeostatic model assessment of insulin resistance (HOMA-IR) was calculated using the formula HOMA-IR = glucose × insulin/400. Fasting serum levels of leptin, adiponectin, resistin, and plasminogen activator inhibitor-1 (PAI-1) were determined by multiplex Luminex assays according to the manufacturer’s instructions (MADKMAG-71K and MADPNMAG-70K-01; MilliporeSigma).
9
Glucose Consumption of Bladder Cancer Cells
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Bladder cancer cells were transfected with the corresponding siRNAs or plasmids and cultured for 36 h, followed by incubation with RPMI 1640 medium without L-glucose and phenol red for an additional 8 h. The amount of glucose in the medium was determined using a glucose colorimetric assay kit (K606-100; BioVision, Milpitas, CA, United States), following the manufacturer’s protocols.
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