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Integrin β1 sirna b

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Integrin β1 siRNA B is a small interfering RNA (siRNA) designed to target and downregulate the expression of the integrin β1 gene. Integrins are a family of cell surface receptors that play a crucial role in cell-cell and cell-extracellular matrix interactions. This siRNA product can be used to investigate the biological functions of integrin β1 in various cell-based studies.

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Integrin β1, by Cell Signaling Technology (2 mentions) Integrin α5, by Cell Signaling Technology (2 mentions) Opti mem, by Thermo Fisher Scientific (2 mentions) Integrin α5 sirna, by Santa Cruz Biotechnology (2 mentions) Fibronectin pool, by Santa Cruz Biotechnology (2 mentions) Integrin β1 sirna, by Santa Cruz Biotechnology (2 mentions) Fibronectin sirna, by Santa Cruz Biotechnology (2 mentions)

Spelling variants of this product

SiRNAs (95 citations) Integrin β1 (24 citations) Integrin β1 siRNA (5 citations) SiRNA-B (3 citations)

2 protocols using integrin β1 sirna b

1

Integrin and Fibronectin Knockdown Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were plated and left to grow overnight in 5% FBS/RPMI. 5%FBS/RPMI media was replaced with Opti-MEM (Invitrogen). Fibronectin pool, Fibronectin siRNA A GACUGGUGGUUACAUGUUAtt, Fibronectin siRNA B CGCAUCACUUGCACUUCUAtt, Fibronectin siRNA C GAUCCUGUCUACUUCACAAtt, Integrin α5 siRNA A GUCAGAAUUUCGAGACAAAtt, Integrin α5 siRNA B CCACUGACCAGAACUAGAAtt, Integrin β1 siRNA A GAGAUGAGGUUCAAUUUGAtt, Integrin β1 siRNA B GAUGAGGUUCAAUUUGAAAtt, (25nM, Santa Cruz), Integrin α5, Integrin β1, and PTEN (all 25nM, Cell Signaling Technologies), and BRAF (25nM, Thermo Scientific) siRNAs in complex with Lipofectamine 2000 (Invitrogen) were added. Scrambled, non-targeting siRNAs were used as controls. A final concentration of 5% FBS in RPMI was added the next day. For fibronectin knockdowns, final concentration 1% FBS in RPMI was added next day. Cells were transfected for 24-72 hours prior to treatment.
Fedorenko I.V., Abel E.V., Koomen J.M., Fang B., Wood E.R., Chen Y.A., Fisher K.J., Iyengar S., Dahlman K.B., Wargo J.A., Flaherty K.T., Sosman J.A., Sondak V.K., Messina J.L., Gibney G.T, & Smalley K.S. (2015). Fibronectin induction abrogates the BRAF inhibitor response of BRAF V600E/PTEN-null melanoma cells. Oncogene, 35(10), 1225-1235.
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2

Integrin and Fibronectin Knockdown Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were plated and left to grow overnight in 5% FBS/RPMI. 5%FBS/RPMI media was replaced with Opti-MEM (Invitrogen). Fibronectin pool, Fibronectin siRNA A GACUGGUGGUUACAUGUUAtt, Fibronectin siRNA B CGCAUCACUUGCACUUCUAtt, Fibronectin siRNA C GAUCCUGUCUACUUCACAAtt, Integrin α5 siRNA A GUCAGAAUUUCGAGACAAAtt, Integrin α5 siRNA B CCACUGACCAGAACUAGAAtt, Integrin β1 siRNA A GAGAUGAGGUUCAAUUUGAtt, Integrin β1 siRNA B GAUGAGGUUCAAUUUGAAAtt, (25nM, Santa Cruz), Integrin α5, Integrin β1, and PTEN (all 25nM, Cell Signaling Technologies), and BRAF (25nM, Thermo Scientific) siRNAs in complex with Lipofectamine 2000 (Invitrogen) were added. Scrambled, non-targeting siRNAs were used as controls. A final concentration of 5% FBS in RPMI was added the next day. For fibronectin knockdowns, final concentration 1% FBS in RPMI was added next day. Cells were transfected for 24-72 hours prior to treatment.
Fedorenko I.V., Abel E.V., Koomen J.M., Fang B., Wood E.R., Chen Y.A., Fisher K.J., Iyengar S., Dahlman K.B., Wargo J.A., Flaherty K.T., Sosman J.A., Sondak V.K., Messina J.L., Gibney G.T, & Smalley K.S. (2015). Fibronectin induction abrogates the BRAF inhibitor response of BRAF V600E/PTEN-null melanoma cells. Oncogene, 35(10), 1225-1235.
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