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Anti gst sc 459

Manufactured by Santa Cruz Biotechnology
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Anti-GST (sc-459) is a primary antibody that recognizes the Glutathione S-Transferase (GST) protein. It is designed for use in various immunological techniques, such as Western Blotting, Immunoprecipitation, and Immunohistochemistry, to detect and study the GST protein in biological samples.

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Lab products found in correlation

Anti trimethylated h3k9, by Active Motif (1 mentions) Anti dimethylated h3k9, by Active Motif (1 mentions) Anti total h3, by Active Motif (1 mentions) Anti phospho s65 4e bp1, by Cell Signaling Technology (1 mentions) Anti phospho t172 ampkα, by Cell Signaling Technology (1 mentions) Hela cell, by American Type Culture Collection (1 mentions) Mv4 11, by American Type Culture Collection (1 mentions) Molm 13, by American Type Culture Collection (1 mentions) Anti β actin a1978 antibody, by Merck Group (1 mentions) Glutathione agarose beads, by Merck Group (1 mentions) Anti ha, by Cell Signaling Technology (1 mentions) Anti lamin b1, by GeneTex (1 mentions) Anti v5, by Cell Signaling Technology (1 mentions) Anti his, by Merck Group (1 mentions) Anti na, by GeneTex (1 mentions) Anti m1, by GeneTex (1 mentions) Anti ns1, by GeneTex (1 mentions) Anti pb2, by GeneTex (1 mentions) Anti α tubulin clone dm1a, by Merck Group (1 mentions) Anti np, by GeneTex (1 mentions)

6 protocols using anti gst sc 459

1

Chromatin Immunoprecipitation Assay Protocol

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ChIP assay was carried out as described previously [21] (link). Chromatin extracts were immunoprecipitated using 10 μg anti-KDM4C (A300-885 A; Bethyl Laboratories); 4 μg anti-trimethylated H3K9 (61,013; Active Motif), 4 μg anti-dimethylated H3K9 (39,683; Active Motif), and 2 μg anti-total H3 (39,763; Active Motif); Anti-GST (sc-459; Santa Cruz Biotechnology) antibodies were used as mock ChIP controls. Fold enrichments were calculated by determining the ratios of the amount of immunoprecipitated DNA to that of the input sample and were normalized to the level observed at a control region, which was defined as 1.0. The ChIP primers are available on request (Supplemental Table Primers).
Yuan X., Kong J., Ma Z., Li N., Jia R., Liu Y., Zhou F., Zhan Q., Liu G, & Gao S. (2016). KDM4C, a H3K9me3 Histone Demethylase, is Involved in the Maintenance of Human ESCC-Initiating Cells by Epigenetically Enhancing SOX2 Expression. Neoplasia (New York, N.Y.), 18(10), 594-609.
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2

Immunoblotting for Autophagy and Signaling

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Anti-LC3B (ab51520), anti-S6K1 (ab32359), anti-phospho-T389 S6K1 (ab2571) and horseradish peroxidase (HRP) -labelled anti-horse IgG secondary antibodies (ab6802) were purchased from Abcam. Anti-GST (sc-459) was purchased from Santa Cruz Biotechnology. Anti-p62 (CST 5114), anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (CST 2118), anti-phospho-S65 4E-BP1 (CST 9451), anti-phospho-S757 ULK1 (CST 6888), anti-phospho-S473 Akt (CST 4058), anti-phospho-T172 AMPKα (CST 2531) and HRP-labelled anti-rabbit IgG secondary antibodies (CST 7074) were purchased from Cell Signaling Technology. His-tagged LRS and GST-tagged RagD were in laboratory stocks. mCherry-GFP-LC3 plasmid (pBabe vector) was from Dr Heesun Cheong, Division of Chemical Biology, Research Institute, National Cancer Center, Korea.
Kim J., Jung J., Koo J., Cho W., Lee W.S., Kim C., Park W, & Park S.B. (2016). Diversity-oriented synthetic strategy for developing a chemical modulator of protein–protein interaction. Nature Communications, 7, 13196.
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3

Histone Peptide Binding Assay for ENL, GAS41, and AF9

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The biotinylated histone peptides used in the AlphaScreen assay: H3 (aa 1–22, ARTKQTARKSTGGKAPRKQLAT), H3K9ac (aa 1–22, ARTKQTARK(ac)STGGKAPR-KQLAT), H3 (aa 21–44, ATKAARKSAPATGGVKKPHRYRPG), H3K27ac (aa 21–44, ATKAARK(ac)SAPSTGGVKKPHRYRPG), H3K27cr (aa 21–44, ATKAARK(cro)SAPS-TGGVKKPHRYRPG) and the biotin-14xHis peptide used in counter assay were purchased from CPC Scientific. Anti-ENL (14893S) antibody was from Cell Signaling, anti-GAS41 (sc-393708) and anti-GST (sc-459) antibodies were from Santa Cruz, and anti-AF9 (HPA001824) and anti-β-actin (A1978) antibody was from Sigma. Human cell lines MV4;11, MOLM13, U2OS and HeLa cells were purchased from ATCC.
Ma X.R., Xu L., Xu S., Klein B.J., Wang H., Das S., Li K., Yang K.S., Sohail S., Chapman A., Kutateladze T.G., Shi X., Liu W.R, & Wen H. (2021). Discovery of Selective Small Molecule Inhibitors for the ENL YEATS Domain. Journal of medicinal chemistry, 64(15), 10997-11013.
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4

GST-HDAC4c Interaction with SRD3c

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GST-tagged HDAC4c was coexpressed with Gal4N-fused SRD3c wild-type or mutants in HEK293 cells. After 48 h, the whole cell lysates (500 μg) were prepared in RIPA buffer [50 mM Tris-HCl (pH 8.0), 5 mM EDTA, 150 mM NaCl, 1% NP-40, 1 mM PMSF] and mixed with 30 μl (50% slurry) of glutathione-agarose beads (Sigma-Aldrich). After overnight incubation at 4°C, the beads were washed three times with the same buffer. The bound proteins were eluted from the precipitates using 2× SDS sample buffer and resolved by 10% SDS-PAGE. The presence of Gal4N-SRD3c proteins and GST- HDAC4c was analyzed by immunoblotting using anti-GBD (sc-510; Santa Cruz Biotechnology, Dallas, TX, USA) and anti-GST (sc-459; Santa Cruz Biotechnology) antibodies, respectively.
Park S.Y., Kim G.S., Hwang H.J., Nam T.H., Park H.S., Song J., Jang T.H., Lee Y.C, & Kim J.S. (2018). Structural basis of the specific interaction of SMRT corepressor with histone deacetylase 4. Nucleic Acids Research, 46(22), 11776-11788.
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5

Antibody panel for protein detection

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The antibodies used in this study included anti-HA (#3724, Cell Signaling), anti-V5 (#13202, Cell Signaling; T0057, Affinity Biosciences), anti-Flag M2 (F3165; Sigma), anti-OTUB1 (GTX57636, GeneTex), anti-lamin-B1 (GTX103292, GeneTex), anti-α-tubulin (Clone DM1A, T9026; Sigma), anti-ubiquitin (Ub) (A11227, Abclonal), anti-PB2 (GTX125926, GeneTex), anti-NP (GTX125989, GeneTex), anti-M1 (GTX125928, GeneTex), anti-NS1 (GTX125990, GeneTex), anti-NA (GTX125974, GeneTex), anti-NS2 (GTX125953, GeneTex), anti-GST (sc-459, Santa Cruz Biotechnology), and anti-His (05-949, Millipore) antibodies.
Li Y.J., Chen C.Y., Kuo Y.S., Huang Y.W., Kuo R.L., Chang L.K., Yang J.H., Lai C.H., Shih S.R, & Chiu Y.F. (2024). OTUB1 contributes to the stability and function of Influenza A virus NS2. PLoS pathogens, 20(5).
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6

Recombinant EGFR Protein Expression

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E. coli strain BL21 CodonPlus (Stratagene) was used for GST-fusion protein expression and JM109 competent bacteria (Promega) were used for plasmid construction and maintenance. The vector pLXSN, containing the full-length human EGFR, was a gift from Professor Axel Ullrich (Max Planck Institute, Martinsried, Germany). E. coli expression vector pGEX-6P-1 was purchased from Amersham Pharmacia Biotech. Anti-EGFR (sc-03) and anti-GST (sc-459) antibodies were obtained from Santa-Cruz Biotechnology. The horseradish peroxidase conjugated anti-rabbit and anti-mouse IgG antibodies were purchased from Promega.
Elloumi-Mseddi J., Jellali K., Villalobo A, & Aifa S. (2014). A Premature Termination of Human Epidermal Growth Factor Receptor Transcription in Escherichia coli. The Scientific World Journal, 2014, 830923.
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