Triazolam (NIDA, Baltimore, MD) was dissolved in 100% propylene glycol and diluted to a 20% propylene glycol/80% water mixture. Clonazepam (Sigma-Aldrich, St. Louis, MO) was dissolved in 100% propylene glycol and diluted to a 50% propylene glycol/50% water mixture. Pregnanolone [(3α,5β)-3-Hydroxy-pregnan-20-one] and ganaxolone [(3α,5α)-3-Hydroxy-3-methyl-pregnan-20-one] (Tocris Biosciences, Ellisville, MO) were dissolved in 45% (w/v) 2-hydroxypropyl-β-cyclodextrin. Morphine was dissolved in saline. All drugs were administered intraperitoneally in volumes of 1–2 ml/kg.
Pregnanolone
Manufactured by Bio-Techne
Sourced in United Kingdom
Pregnanolone is a neurosteroid compound that can be used in laboratory research applications. It is a naturally occurring metabolite of progesterone. Pregnanolone functions as a positive allosteric modulator of the GABA-A receptor.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using pregnanolone
1
Intranasal Administration of Neurosteroids
2
Drug Combination Preparation for Behavioral Studies
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Midazolam (Hospira Inc., Lake Forest, IL, United States) 5 mg/ml pharmaceutical stock was diluted with 0.9% saline solution. Triazolam (Sigma-Aldrich, St. Louis, MO, United States) was dissolved in propylene glycol and diluted with sterile water to a 50% propylene glycol/50% sterile water solution. Pregnanolone (Tocris Bioscience, Bristol, United Kingdom) was dissolved in a 45% (w/v) 2-hydroxypropyl-β-cyclodextrin solution. Triazolam-Pregnanolone combinations for the self-administration study were prepared by dissolving each drug separately at twice the concentration of the test dose. The separate solutions were then combined in a single syringe prior to test sessions to create the test combination. Triazolam-Pregnanolone combinations for the observation study were prepared by dissolving each drug separately and then administered sequentially via the i.v. catheter. In these sessions, the test dose of Triazolam was administered first, followed by the test dose of Pregnanolone.
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