Owl easycast b1a mini gel system

Manufactured by Thermo Fisher Scientific

The Owl EasyCast B1A mini gel system is a compact and convenient electrophoresis unit designed for the separation and analysis of DNA, RNA, or protein samples. It provides a streamlined solution for performing basic gel electrophoresis in the laboratory.

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Lab products found in correlation

2 2 2 trichloroethanol, by Thermo Fisher Scientific (2 mentions) Chemidoc mp imaging system, by Bio-Rad (2 mentions) Tae buffer, by Thermo Fisher Scientific (1 mentions)

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Owl EasyCast B1A Mini-gels

2 protocols using owl easycast b1a mini gel system

Detecting Amyloid Aggregates by SDD-AGE

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To confirm large aggregate/fibril formation, SDD-AGE was used. Standard agarose (1.5%) was melted in 1X Tris acetate EDTA (TAE) buffer (Fisher Scientific) combined with 0.1% SDS, 0.5 μM 2,2,2-trichloroethanol (Alfa Aesar) for stain-free detection and 1 μM ThT for fibril detection (Sigma). The hot solution was poured into an Owl EasyCast B1A mini gel system (Thermo Fisher Scientific). NANOG WR domain and CTD gel samples were first formed by diluting stock samples in 7 M GdnHCl to PBS buffer. The gel/precipitates were resuspended in 6X sample buffer (5:1) and pipetted several times before 5 min of r.t. incubation. Electrophoresis was performed in 1X TAE containing 0.1% SDS for 90 min at 80 V. After electrophoresis, the gel was imaged using stain-free and Alexa 488 filters on the ChemiDoc MP imaging system (Bio-Rad). Bovine serum albumin (BSA; 3 and 10 μg) was used as negative control.

Choi K.J., Quan M.D., Qi C., Lee J.H., Tsoi P.S., Zahabiyon M., Bajic A., Hu L., Prasad B.V., Liao S.C., Li W., Ferreon A.C, & Ferreon J.C. (2022). NANOG prion-like assembly mediates DNA bridging to facilitate chromatin reorganization and activation of pluripotency. Nature Cell Biology, 24(5), 737-747.

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SDD-AGE for Aggregates and Fibrils Detection

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To confirm large aggregate/fibril formation, SDD-AGE was employed. Standard agarose (1.5%) was melted in a 1X TAE buffer (Fisher scientific) combined with 0.1% SDS, 0.5 μM 2,2,2-Trichloroethanol (Alfa Aesar) for stain-free detection and 1 μM Thioflavin T for fibril detection (Sigma). The hot solution was poured into an Owl EasyCast B1A mini gel system (Thermo Fisher Scientific). NANOG WR domain and CTD gel samples were first formed by diluting stock samples in 7 M GdnHCl to PBS buffer. The gel/precipitates were resuspended in 6X sample buffer (5:1) and pipetted for several times before 5 min RT incubation. Electrophoresis was performed in 1X TAE containing 0.1% SDS for 90 min at 80 V. After electrophoresis, the gel was imaged using the stain-free and Alexa 488 filters on the ChemiDoc MP imaging system (Bio-Rad). 3 and 10 μg BSA were used as negative controls.

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