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Rabbit anti gldc hpa002318

Manufactured by Merck Group
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Rabbit anti-GLDC (HPA002318) is an antibody product from Merck Group, designed for use in laboratory research applications. The antibody specifically targets the GLDC protein, which is involved in glycine decarboxylase complex activity. This product can be used for techniques such as immunohistochemistry and Western blotting to detect and analyze the GLDC protein in biological samples.

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Lab products found in correlation

Ab33911, by Abcam (2 mentions) Nitrocellulose membrane, by LI COR (2 mentions) Protein assay dye, by Bio-Rad (2 mentions) Mouse anti α tubulin t5168, by Merck Group (2 mentions) Anti rabbit irdye 800, by LI COR (2 mentions) Rabbit anti gapdh sc 25778, by Santa Cruz Biotechnology (2 mentions) Goat anti rabbit igg, by Santa Cruz Biotechnology (2 mentions) Rabbit anti myc sc 764, by Santa Cruz Biotechnology (2 mentions) Horseradish peroxidase conjugated goat anti mouse sc 2005, by Santa Cruz Biotechnology (2 mentions) Odyssey system, by LI COR (2 mentions) Mouse anti mycn clone op13, by Merck Group (2 mentions)

Close spelling variants of this product

Rabbit anti- (2 citations)

2 protocols using rabbit anti gldc hpa002318

1

Western Blot Analysis of Cell Lysates

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Cells were lysed using SDS sample buffer and protein concentrations were measured using Bio-Rad Protein Assay Dye (Bio-Rad 5000006). Proteins (~50 μg per sample) were separated on SDS-polyacrylamide gels, transferred to nitrocellulose membranes (926–31092, LI-COR Biosciences, Lincoln, NE), and probed with rabbit anti-cyclin E1 (ab33911, Abcam, Cambridge, MA), rabbit anti-GLDC (HPA002318, Sigma-Aldrich), mouse anti-MYCN (clone OP13, Millipore Sigma), rabbit anti-MYC (sc-764, Santa Cruz Biotech, Dallas, TX), rabbit anti-GAPDH (sc-25778, Santa Cruz Biotech), and mouse anti-α-tubulin (T5168, Sigma-Aldrich) antibodies. Horseradish Peroxidase conjugated goat anti-mouse (sc-2005, Santa Cruz Biotech) and goat anti-rabbit IgG (sc2004, Santa Cruz Biotech) were used as secondary antibodies to visualize proteins by chemiluminescence. For visualization with the Odyssey system (LI-COR Biosciences), Goat anti-mouse IRDye 800 (926–32210) or 680 (926–32220) and anti-rabbit IRDye 800 (926–32234) or 680 (926–68021) from LI-COR Biosciences were used as secondary antibodies.
Alptekin A., Ye B., Yu Y., Poole C.J., van Riggelen J., Zha Y, & Ding H.F. (2019). Glycine decarboxylase is a transcriptional target of MYCN required for neuroblastoma cell proliferation and tumorigenicity. Oncogene, 38(50), 7504-7520.
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2

Western Blot Analysis of Cell Lysates

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed using SDS sample buffer and protein concentrations were measured using Bio-Rad Protein Assay Dye (Bio-Rad 5000006). Proteins (~50 μg per sample) were separated on SDS-polyacrylamide gels, transferred to nitrocellulose membranes (926–31092, LI-COR Biosciences, Lincoln, NE), and probed with rabbit anti-cyclin E1 (ab33911, Abcam, Cambridge, MA), rabbit anti-GLDC (HPA002318, Sigma-Aldrich), mouse anti-MYCN (clone OP13, Millipore Sigma), rabbit anti-MYC (sc-764, Santa Cruz Biotech, Dallas, TX), rabbit anti-GAPDH (sc-25778, Santa Cruz Biotech), and mouse anti-α-tubulin (T5168, Sigma-Aldrich) antibodies. Horseradish Peroxidase conjugated goat anti-mouse (sc-2005, Santa Cruz Biotech) and goat anti-rabbit IgG (sc2004, Santa Cruz Biotech) were used as secondary antibodies to visualize proteins by chemiluminescence. For visualization with the Odyssey system (LI-COR Biosciences), Goat anti-mouse IRDye 800 (926–32210) or 680 (926–32220) and anti-rabbit IRDye 800 (926–32234) or 680 (926–68021) from LI-COR Biosciences were used as secondary antibodies.
Alptekin A., Ye B., Yu Y., Poole C.J., van Riggelen J., Zha Y, & Ding H.F. (2019). Glycine decarboxylase is a transcriptional target of MYCN required for neuroblastoma cell proliferation and tumorigenicity. Oncogene, 38(50), 7504-7520.
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