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Magmax rna isolation system

Manufactured by Thermo Fisher Scientific
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The MagMax RNA Isolation System is a magnetic bead-based technology designed for the automated extraction and purification of RNA from a variety of sample types. The core function of this system is to provide a standardized and efficient method for isolating high-quality RNA for downstream analysis and applications.

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Lab products found in correlation

Magmax 96 total rna isolation kit, by Thermo Fisher Scientific (2 mentions) Quantitect sybr green pcr kit, by Qiagen (2 mentions) Superscript vilo cdna synthesis kit, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

MagMAX system (2 citations) MagMax total RNA isolation system (2 citations)

2 protocols using magmax rna isolation system

1

RT-qPCR Analysis of hESC and HIO

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA from hESC day 0 spheroids or HIOs grown in PEG-4MAL hydrogels or Matrigel™ was extracted using the MagMax RNA isolation system and MagMax-96 total RNA isolation Kit (AM1830, ThermoFisher Scientific). cDNA was synthesized using the SuperScript VILO cDNA Synthesis Kit (11754-250, ThermoFisher Scientific). RT-qPCR was carried out using the QuantiTect SYBR Green PCR Kit (204145, Qiagen). Relative gene expression was plotted as Arbitrary Units using the formula: [2ˆ(housekeeping gene Ct – gene of interest Ct)] × 10,000. Primer sequences for RT-qPCR are provided in Supplementary Table 2.
Cruz-Acuña R., Quirós M., Farkas A.E., Dedhia P.H., Huang S., Siuda D., García-Hernández V., Miller A.J., Spence J.R., Nusrat A, & García A.J. (2017). Synthetic Hydrogels for Human Intestinal Organoid Generation and Colonic Wound Repair. Nature cell biology, 19(11), 1326-1335.
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2

RT-qPCR Analysis of hESC and HIO

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA from hESC day 0 spheroids or HIOs grown in PEG-4MAL hydrogels or Matrigel™ was extracted using the MagMax RNA isolation system and MagMax-96 total RNA isolation Kit (AM1830, ThermoFisher Scientific). cDNA was synthesized using the SuperScript VILO cDNA Synthesis Kit (11754-250, ThermoFisher Scientific). RT-qPCR was carried out using the QuantiTect SYBR Green PCR Kit (204145, Qiagen). Relative gene expression was plotted as Arbitrary Units using the formula: [2ˆ(housekeeping gene Ct – gene of interest Ct)] × 10,000. Primer sequences for RT-qPCR are provided in Supplementary Table 2.
Cruz-Acuña R., Quirós M., Farkas A.E., Dedhia P.H., Huang S., Siuda D., García-Hernández V., Miller A.J., Spence J.R., Nusrat A, & García A.J. (2017). Synthetic Hydrogels for Human Intestinal Organoid Generation and Colonic Wound Repair. Nature cell biology, 19(11), 1326-1335.
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