Image studio imaging software

Manufactured by LI COR

Image Studio is a software application that provides image capture, analysis, and quantification capabilities for LI-COR instruments. It supports a range of imaging techniques and enables users to process, visualize, and interpret data from various experiments.

Automatically generated - may contain errors

Lab products found in correlation

4 protocols using image studio imaging software

SLO Detection via Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols

CSPs were separated by SDS-PAGE and transferred to nitrocellulose membranes (Amersham; 0.45 μm). For the detection of SLO, a monoclonal anti-streptolysin O antibody (Abcam) was diluted 1:5,000 in 5% nonfat milk and incubated with the membranes for 1 h at room temperature. The membranes were then washed with a solution of phosphate-buffered saline (PBS)–Tween (0.1%) 3 times for 5 min each. The primary antibody was detected using an anti-mouse IgG secondary antibody conjugated with IRDye 700 dye (Li-Cor), which was diluted 1:10,000 in 5% nonfat milk and incubated with the membrane blots for 1 h. The blots were washed and analyzed with densitometry using an Odyssey CLx infrared imaging system with Image Studio imaging software (Li-Cor). Densitometry values (expressed in arbitrary units [AU]) were obtained using the Image Studio imaging software associated with the Li-Cor Odyssey infrared imaging system. To confirm that equal amounts of protein were analyzed, a separate SDS-PAGE gel was stained for total protein using Sypro ruby protein gel stain (Bio-Rad).

Herrera A.L., Callegari E.A, & Chaussee M.S. (2021). The Streptococcus pyogenes Signaling Peptide SpoV Regulates Streptolysin O and Enhances Survival in Murine Blood. Journal of Bacteriology, 203(11), e00586-20.

+ Open protocol

+ Expand

Quantification of Chlamydia Protein Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols

siRNA transfected cells were infected with Chlamydia as described above. Samples were separated on an SDS-12%PAGE and transferred using an iBlot transfer apparatus (Life Technologies). Primary antibodies used in this study include: rabbit anti-syntaxin 10 (Abgent; SanDiego, CA), mouse anti-GAPDH (EMD Millipore; Darmstadt, Germany), rabbit anti-Hc1 (Ted Hackstadt), rabbit anti-OmcB (Thomas Hatch, University of Tennessee Health Science Center, Memphis, TN), and mouse anti-HSP60 (Rick Morrison, Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, AR). The primary antibodies were detected using appropriate anti-mouse or anti-rabbit IgG secondary antibodies conjugated with IRDye 700 or IRDye 800 dyes (LI-COR). The blots were scanned and analyzed by densitometry with an Odyssey Infrared Imaging System using Image Studio imaging software (LI-COR). To quantitate OmcB and Hc1 protein levels, densitometry values were normalized to cHSP60, which was first normalized to host GAPDH. Data are representative of two independent experiments and results are expressed as mean and standard error of the mean, calculated by GraphPad Prism 6 software.

Lucas A.L., Ouellette S.P., Kabeiseman E.J., Cichos K.H, & Rucks E.A. (2015). The trans-Golgi SNARE syntaxin 10 is required for optimal development of Chlamydia trachomatis. Frontiers in Cellular and Infection Microbiology, 5, 68.

+ Open protocol

+ Expand

Western Blot Analysis of Protein Lysates

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cell lysates were prepared using previously reported methods [38 (link)–41 (link)]. Briefly, cells were lysed in M-PER (Mammalian Protein Extraction Reagent, Thermo Fisher scientific) with 1X Halt protease inhibitor cocktail (Thermo Fisher Scientific). The lysates were collected, centrifuged at 10,000 g for 20-min, pellets were discarded, and supernatants were transferred to a new Eppendorf tube. Then, samples were analyzed for protein concentration and an equal amount of protein was applied to electrophoresis, separated on 4–12% gradient Bis-Tris SDS-PAGE gels (Novex, Life Technologies), and transferred to PVDF membranes (Novex, Life Technologies). Subsequently, the membranes were blocked for 30-min with SuperBlock™ (Thermo Fisher Scientific) and probed with primary antibodies, including G12A (1:2000), BACE1 (1:1000) and β-actin (1:1000), overnight at 4 °C. The membranes were then washed 5 times with TBS-T and incubated with appropriate secondary antibodies for 1-h at room temperature. After 5 additional washing steps, the Femto SuperSignal™ Substrate (Life Technologies) was used to develop the membranes. The membranes were developed by the Odyssey® Fc and analyzed by the Image Studio imaging software (LI-COR, Inc.).

Liang Y., Raven F., Ward J.F., Zhen S., Zhang S., Sun H., Miller S.J., Choi S.H., Tanzi R.E, & Zhang C. (2020). Upregulation of Alzheimer’s amyloid-β protein precursor in astrocytes both in vitro and in vivo. Journal of Alzheimer's disease : JAD, 76(3), 1071-1082.

+ Open protocol

+ Expand

Syntaxin 10 Knockdown Impact on Chlamydia

Check if the same lab product or an alternative is used in the 5 most similar protocols

Silencer select siRNA (Life Technologies) against syntaxin 10 (Stx10); (s16535) and non-targeting control (NT); (4390843) were used at a final concentration of 10 nM. All siRNA clones were validated by Life Technologies according to their procedures. Three different siRNA clones against Stx10 were used (ID number-137195, ID number-137196, and ID number-137197) to quantify chlamydial infectious progeny at 44 h of infection. Based on similar results between all Stx10 siRNAs (data not shown), Stx10 ID number-137197 was used for all subsequent siRNA experiments. siRNA was delivered to HeLa cells via reverse transfection using Lipofectamine RNAiMAX siRNA Transfection Reagent and Opti-MEM media (Life Technologies), following the manufacturer's protocol. For all siRNA knockdown experiments, monolayers were infected with C. trachomatis serovar L2 48 h after siRNA transfection. Efficiency of knockdown was confirmed by Western blot and densitometry analysis using Odyssey Infrared Imaging System using Image Studio imaging software (LI-COR, Lincoln, NE). Only samples achieving 70% or greater knockdown efficiency were used in subsequent studies.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!