E6 thermal imaging infrared camera

Body composition (fat mass, lean mass and fluid) was assessed by using a magnetic resonance whole-body composition analyzer (Minispec LF90 II, Bruker, Karlsruhe, Germany). Oxygen consumption, carbon dioxide production and locomotor activity were measured using a sixteen-chamber indirect calorimeter (TSE PhenoMaster) according to the manufacturer’s instructions [56 (link)]. The mice were maintained at 22 °C under a 12 h light-dark cycle following a 12 h acclimatization. To obtain metabolic parameters under cold stimuli, mice were intraperitoneally injected with the β3-adrenergic receptor-specific agonist CL316,243 (1 mg/kg) after a 12 h light/dark cycle. The mice were given free access to water and food. Heat production as name as energy expenditure, oxygen expenditure and activity were calculated as described previously. For cold exposure, mice were single-caged and exposed to a temperature of 4 °C for 6 h. Rectal temperature was monitored every hour using a BAT-12 microprobe digital thermometer and RET-3 mouse rectal probe (Physitemp Instruments, Clifton, NJ, USA). Thermo images were taken using an E6 Thermal Imaging Infrared Camera (E6, FLIR Systems, Portland, OR, USA) at 6 h, and the interscapular skin temperature was analyzed using FLIR Tools software.