Sodium carbonate anhydrous (Na2CO3), Folin-Ciocalteu reagent, sodium nitrite solution (NaNO2), aluminum chloride hexahydrate solution (AlCl3, 6 H2O), vanillin, catechin and gallic acid were purchased from Fluka (Buchs, Switzerland). Sulfuric acid (H2SO4) was obtained from Merck (Darmstadt, Germany). Fluorescein sodium salt (FL), 20,70 dichlorofluorescin-diacetate (DCFH-DA), 20,70-dichlorofluorescin (DCFH), 20,70-dichlorofluorescein (DCF), 6-hydroxy-2,5,7,8-tetramethyl-2-carboxylic acid, tert-butyl hydroperoxide (t-BuOOH), (Trolox), quercetin, and 2,20-azobis(2-amidino-propane) dihydrochloride (AAPH) were all purchased from Sigma-Aldrich (Oakville, ON). All the solvents were purchased from EMD (Canada).
20 70 dichlorofluorescin diacetate
Manufactured by Merck Group
Sourced in Ireland
20,70 dichlorofluorescin-diacetate is a fluorogenic compound used in biological research. It is a cell-permeable indicator for reactive oxygen species. Upon cleavage by esterases and oxidation, 20,70 dichlorofluorescin-diacetate produces a fluorescent derivative, which can be detected and quantified.
Automatically generated - may contain errors
Lab products found in correlation
Fluorescein sodium salt, by Merck Group (1 mentions)
Quercetin, by Merck Group (1 mentions)
Sulfuric acid h2so4, by Merck Group (1 mentions)
6 hydroxy 2 5 7 8 tetramethyl 2 carboxylic acid, by Merck Group (1 mentions)
20 70 dichlorofluorescein, by Merck Group (1 mentions)
2 20 azobis 2 amidino propane dihydrochloride aaph, by Merck Group (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Znonps nanopowder, by Merck Group (1 mentions)
2 protocols using 20 70 dichlorofluorescin diacetate
1
Antioxidant Capacity Evaluation Protocol
2
Nanoparticle Cytotoxicity Screening Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Ag NPs Nano powder water dispersion (Ag, 15 nm) were purchased from US Research Nanomaterial, Inc. Latex beads, AmPs NPs (50 nm mean particle size), ZnO NPs Nano powder (ZnO, <100 nm particle size), 3-(4,5-Dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT), Neutral Red (NR), Propidium Iodide (PI), Doxorubicin hydrochloride V R (DOX), and 2 0 ,7 0 -dichlorofluorescin diacetate (DCF) as well as all cell culture media, supplements, the trypsinisation solution were all purchased from Sigma Aldrich Ltd. (Ireland). AB, Cell event Caspase 3/7 Green Flow Cytometry Kit and KaryoMax Colcemid Solution in HBBS were all purchased from Biosciences (Dublin, Ireland).
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