Log In Sign up
The largest database of trusted experimental protocols

Hdl and ldl vldl quantitation kit

Manufactured by Merck Group
Visit Supplier
Sourced in United States

The HDL and LDL/VLDL Quantitation Kit is a laboratory equipment product designed to quantify the levels of high-density lipoprotein (HDL) and low-density lipoprotein (LDL)/very-low-density lipoprotein (VLDL) in biological samples. The kit provides the necessary reagents and protocols to perform these measurements.

Automatically generated - may contain errors

Lab products found in correlation

Il 10, by BioLegend (1 mentions) Tgf β, by Thermo Fisher Scientific (1 mentions) Dipotassium edta, by BD (1 mentions) Elisa assay, by BioLegend (1 mentions) Microtainer tube, by BD (1 mentions) Bile acid assay kit, by Merck Group (1 mentions) Infinity triglycerides reagent, by Thermo Fisher Scientific (1 mentions) Infinity cholesterol reagent, by Thermo Fisher Scientific (1 mentions) Porcine insulin elisa kit, by Mercodia (1 mentions) Free fatty acid quantitation kit, by Merck Group (1 mentions) Spotchem sp 4410, by Arkray (1 mentions) Enzychrom triglyceride assay kit, by BioAssay Systems (1 mentions) Il 10 elisa, by Thermo Fisher Scientific (1 mentions) Apoe b6.129p2 apoetm1unc j, by Jackson ImmunoResearch (1 mentions) C57bl 6j, by Jackson ImmunoResearch (1 mentions) Labassaytm glucose, by Fujifilm (1 mentions) Transaminase cii test, by Fujifilm (1 mentions) Lab assaytm cholesterol, by Fujifilm (1 mentions) Labassaytm triglyceride, by Fujifilm (1 mentions)

10 protocols using hdl and ldl vldl quantitation kit

1

Cholesterol and Cytokine Analysis in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
After 8 weeks treatment, mice were euthanized, and blood was collected by retro-orbital puncture with BD Microtainer tubes and dipotassium EDTA (BD Biosciences). Serum was separated by centrifugation at 3000 rpm at 4°C for 25min and stored at −80C. Total cholesterol was determined by HDL and LDL/VLDL Quantitation Kit (Sigma). Cytokines TGF-β (ThermoFischer Scientific) and IL-10 (Biolegend) were measured by ELISA assays (BioLegend) and cytokines (IL-6, IFN-γ) were measured by a customized Luminex Multiplex panel, according to the manufacturer’s instructions (ThermoFisher Scientific).
Yi S., Zhang X., Sangji H., Liu Y., Allen S.D., Xiao B., Bobbala S., Braverman C.L., Cai L., Hecker P.I., DeBerge M., Thorp E.B., Temel R.E., Stupp S.I, & Scott E.A. (2019). Surface engineered polymersomes for enhanced modulation of dendritic cells during cardiovascular immunotherapy. Advanced functional materials, 29(42), 1904399.
+ Open protocol
+ Expand
2

Quantifying Lipid and Bile Acid Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total hepatic cholesterol and serum HDL- and LDL-cholesterol were measured using HDL and LDL/VLDL Quantitation Kit (MAK045; Sigma). Serum total and fecal bile acid were measured using Bile Acid Assay Kit (MAK309; Sigma). All experiments were performed according to the manufacturer’s instructions. For fecal bile acid, the feces from individually housed mouse were collected over a 72 hr period.
, & Kim H. (2016). The transcription cofactor CRTC1 protects from aberrant hepatic lipid accumulation. Scientific Reports, 6, 37280.
+ Open protocol
+ Expand
3

Serum Cholesterol Fractionation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Serum total cholesterol concentration was determined using a routine method, at the Central Clinical Laboratory, Medical University of Gdansk. Serum HDL- and LDL- + VLDL-cholesterol was measured using HDL and LDL/VLDL Quantitation Kit (Sigma, MAK045). Serum samples were mixed with Precipitation Buffer (2:1), incubated for 10 min at room temperature and centrifuged at 2000 × g for 10 min. The precipitate contains LDL/VLDL and the supernatant HDL fractions of cholesterol. Sediment was collected and centrifuged again to remove all remaining trace HDL supernatant. Precipitate was resuspended in Phosphate Buffered Saline (PBS) and the rest of the experiment was performed according to the manufacturer instruction.
Dettlaff-Pokora A., Sucajtys-Szulc E, & Sledzinski T. (2018). Up-regulation of PCSK9 gene expression and diminished level of LDL-receptor in rat liver as a potential cause of post-lipectomy hypercholesterolemia. Molecular and Cellular Biochemistry, 455(1), 207-217.
+ Open protocol
+ Expand
4

Plasma Lipid Profiling in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Blood were collected from tail tips after different fasting/feeding
conditions as specified, and plasma were further isolated via
centrifugation. Triglyceride and total cholesterol levels were measured
using Infinity Triglycerides Reagent (Thermo Fisher) and Infinity
Cholesterol Reagent (Thermo Fisher) respectively according to the
manufacturer’s instructions. For fast protein liquid chromatography
analysis, pooled plasma samples from mice fasted 16 hours was subjected to
FPLC. Cholesterol was measured in the eluted fractions using Infinity
Cholesterol Reagent (Thermo Fisher). For apolipoprotein analysis, equal
volume of FPLC fractions were subjected to SDS-PAGE and immunoblotting
against APOB. HDL and LDL/VLDL Quantitation Kit (Sigma-Aldrich) was used to
separate HDL from LDL/VLDL in plasma followed by measurement of cholesterol
and TG levels in those fractions.
Yu H., Rimbert A., Palmer A.E., Toyohara T., Xia Y., Xia F., Ferreira L.M., Chen Z., Chen T., Loaiza N., Horwitz N.B., Kacergis M.C., Zhao L., Soukas A.A., Kuivenhoven J.A., Kathiresan S, & Cowan C.A. (2019). GPR146 Deficiency Protects Against Hypercholesterolemia and Atherosclerosis. Cell, 179(6), 1276-1288.e14.
+ Open protocol
+ Expand
5

LDL and sICAM-1 Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
The concentration of LDL was measured in animal serum samples with commercial HDL and LDL/VLDL Quantitation Kit according to the manufacturer's protocol (detection range: 2–10 µg/ml; Sigma-Aldrich, St. Louis, Michigan, USA). The concentration of soluble ICAM-1 (sICAM-1) was measured in animal serum samples using commercial Guinea pig sICAM-1/CD54 ELISA Kit assay as recommended by the manufacturer (detection range: 0.313–20 ng/ml; sensitivity min: 0.188 ng/ml, max: 20 ng/ml; MyBioSource, San Diego, California, USA).
Tomaszewska A., Gonciarz W., Rechcinski T., Chmiela M., Kurdowska A.K, & Krupa A. (2024). Helicobacter pylori components increase the severity of metabolic syndrome and its hepatic manifestations induced by a high fat diet. Scientific Reports, 14, 5764.
+ Open protocol
+ Expand
6

Biochemical Profiling of Serum Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Blood samples were collected from the jugular vein and incubated for 1 h at room temperature to allow clotting. Serum was collected by centrifugation at 5000×g for 10 min and stored at − 80 °C until analyzed. Serum glucose, triglyceride, total cholesterol, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and uric acid levels were assayed using a SPOTCHEM SP-4410 automatic dry chemistry analyzer (ARKRAY, Inc., Kyoto, Japan). Serum insulin levels were measured using porcine insulin ELISA kit (Mercodia AB, Uppsala, Sweden). Serum high density lipoprotein cholesterol (HDL-c) and low and very low density lipoprotein cholesterol (LDL/VLDL-c) were measured using the HDL and LDL/VLDL quantitation kit (Sigma-Aldrich, St. Louis, MO, USA). Serum non-esterified fatty acid (NEFA) was measured using the free fatty acid quantitation kit (Sigma-Aldrich).
Hsu M.C., Wang M.E., Jiang Y.F., Liu H.C., Chen Y.C, & Chiu C.H. (2017). Long-term feeding of high-fat plus high-fructose diet induces isolated impaired glucose tolerance and skeletal muscle insulin resistance in miniature pigs. Diabetology & Metabolic Syndrome, 9, 81.
+ Open protocol
+ Expand
7

Measuring Plasma Lipid Profiles

Check if the same lab product or an alternative is used in the 5 most similar protocols
Plasma triglycerides and cholesterol were measured using the EnzyChrom™ Triglyceride Assay Kit (BioAssay Systems) and the HDL and LDL/VLDL Quantitation Kit (Sigma Aldrich), respectively, as per the manufacturers’ protocols.
Pan Y., Ke H., Yan Z., Geng Y., Asner N., Palani S., Munirathinam G., Dasari S., Nitiss K.C., Bliss S., Patel P., Shen H., Reardon C.A., Getz G.S., Chen A, & Zheng G. (2016). The western-type diet induces anti-HMGB1 autoimmunity in Apoe−/− mice. Atherosclerosis, 251, 31-38.
+ Open protocol
+ Expand
8

In Vivo and Ex Vivo IL-10 Binding

Check if the same lab product or an alternative is used in the 5 most similar protocols
For in vivo binding, very low- and low-density lipoprotein [(V)LDL] were separated from the plasma above using a HDL and LDL/VLDL Quantitation Kit (Sigma-Aldrich) according to manufacturer’s instructions. For ex vivo binding, naïve plasma was incubated with WT IL-10 or Fab-IL-10 constructs (500 ng ml−1) for 2 hr at room temperature. In both cases, the amount of IL-10 in the HDL-rich fraction and the (V)LDL-rich fraction was quantified with an IL-10 ELISA (Invitrogen) and used to calculate the fraction of bound IL-10. Healthy human plasma was purchased from Lonza Biosciences.
Volpatti L.R., de Matos S.N., Borjas G., Reda J., Watkins E.A., Zhou Z., Nguyen M., Solanki A., Fang Y, & Hubbell J.A. (2024). LDL-Binding IL-10 Reduces Vascular Inflammation in Atherosclerotic Mice. bioRxiv.
+ Open protocol
+ Expand
9

Atherosclerosis in ApoE-/- Mouse Model

Check if the same lab product or an alternative is used in the 5 most similar protocols
C57BL/6J and ApoE-/- (B6.129P2-Apoetm1Unc/J) mice were purchased from The Jackson Laboratory. ApoE-/- Aid-/- double-knockout mice were generated in our mouse colony. Male mice were raised on chow diet until they reached full adult maturity at 12 weeks of age and switched to high-fat diet (#D12079B, Research Diets Inc., 21% fat and 0.15% cholesterol) until 28 weeks of age to exacerbate plaque formation. Only male mice were used to eliminate any effects of sex on disease severity (28 (link)). Cholesterol levels were measured from animals that fasted overnight using an HDL and LDL/VLDL Quantitation Kit (Sigma) per manufacturer’s recommendations. All animal protocols were reviewed and approved by the Animal Care and Use Committee of the National Institute on Aging.
Hutchinson M.A., Park H.S., Zanotti K.J., Alvarez-Gonzalez J., Zhang J., Zhang L., Telljohann R., Wang M., Lakatta E.G., Gearhart P.J, & Maul R.W. (2021). Auto-Antibody Production During Experimental Atherosclerosis in ApoE-/- Mice. Frontiers in Immunology, 12, 695220.
+ Open protocol
+ Expand
10

Serum Biochemical Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Serum levels of biochemical parameters, glucose, cholesterol, triglyceride, and AST/ALT were measured using LabAssayTM Glucose, LabAssayTM Cholesterol, LabAssayTM Triglyceride, and Transaminase CII-Test Wako. These kits were from FUJI Film Wako Shibayagi Cooperation (Shibukawa, Japan). Serum levels of HDL and LDL cholesterols were measured using HDL and LDL/VLDL Quantitation Kit (MAK045, Sigma-Aldrich, St. Louis, MO, USA).
He C., Miyazawa T., Abe C., Ueno T., Suzuki M., Mizukami M., Kurihara K, & Toda M. (2023). Hypolipidemic and Anti-Inflammatory Effects of Curcuma longa-Derived Bisacurone in High-Fat Diet-Fed Mice. International Journal of Molecular Sciences, 24(11), 9366.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!