For pharmacological inactivation, ≈500-μm-diameter craniotomies were drilled over cool (32–22 °C)-responsive areas in S1 and pIC in mice anaesthetized with isoflurane (1.5–2% in O2). Both regions were identified functionally using the widefield calcium imaging response to thermal stimuli. Following the craniotomy, the dura was covered with transparent silicone gel (3-4680, Dow Corning). A 300 nl volume of muscimol or Ringer’s solution was injected at a rate of 100 nl min−1 300–500 μm below the pial surface using a pulled glass pipette and a hydraulic injection system (MO-10, Narashige). muscimol (Abcam, ab120094) was dissolved in Ringer’s solution to a concentration of 5 mM. Imaging sessions were carried out >10 min following the end of Ringer’s solution or muscimol injection (Fig. 1f,g ).
Ab120094
Manufactured by Abcam
Ab120094 is a lab equipment product. It is a tool used in scientific research and laboratory settings. The core function of this product is to assist in various experimental procedures. No further details about its intended use or application can be provided in an unbiased and factual manner.
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Lab products found in correlation
2 protocols using ab120094
1
Transient Pharmacological Inactivation of Mouse Somatosensory Cortex
2
Inactivating Mouse Forepaw M1 with Muscimol
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After training mice to perform the triggered reaching task, we performed 2 × 50 nL injections of muscimol (10 mM, AB120094, Abcam) in the identified forepaw M1 at 900 μm and 500 μm depth. Injections were made just before the start of the behavioral testing session. Ringer’s solution was injected as a control, while muscimol was injected on subsequent testing sessions. As a further control, a similar muscimol injection was performed in primary visual cortex (V1). Recovery of behavior was assessed 1 day after M1 muscimol injection.
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