For phenotype identification, BM-MSCs were digested with 0.25% trypsin containing 0.53 mM EDTA and washed with phosphate-buffered saline (PBS). After incubation with antibodies against CD29, CD73, CD90, CD105, CD14, CD34, CD45, and HLA-DR (BD Pharmingen, Franklin Lakes, NJ, USA), the phenotype of BM-MSCs was detected using a BD Influx cell sorter (BD Biosciences, San Jose, CA, USA). For the detection of mIL-6R, BM-MSCs were incubated with anti-human IL-6R antibody (R&D Systems, Minneapolis, MN, USA), and mIL-6R+ cells were detected by flow cytometry.
Anti human il 6r antibody
Manufactured by R&D Systems
Sourced in United States
The Anti-human IL-6R antibody is a laboratory research reagent used to detect and quantify the presence of the human interleukin-6 receptor (IL-6R) in various biological samples. It is a highly specific and sensitive tool for researchers studying the IL-6 signaling pathway and its role in various biological processes and disease states.
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Lab products found in correlation
2 protocols using anti human il 6r antibody
1
Phenotypic Characterization of BM-MSCs
2
IL-6R Staining on 786-O Cells
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For IL-6R staining on 786-O cells a Dako-REAL-EnVision HRP system was used (Dako, Hamburg, Germany). Cells were acetone fixed, peroxidase blocked and stained using an antihuman IL-6R antibody (1:25, R&D systems, Minneapolis, MN, USA) for one hour at room temperature. Second antibody (ready to use) and DAB staining was performed according to the manufacturer's instructions. Cells were HE counterstained. The primary antibody was omitted as negative control.
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