Cell lysates were separated by 10% SDS-PAGE. The specific primary antibodies used in Western blotting analysis are as follows: FAP (1:1000, ab53066, Abcam, UK), α-SMA (1:1000, ab5694, Abcam), IL11 (1:1000, ab89887, Abcam), IL15 (1:800, ab7213, Abcam); VEGFA (1:1000, wl00009b, Wanleibio, China); p-VEGFR2 (1:800, D155165, Sangon, China), T-VEGFR2 (1:1000, D151118, Sangon), p-KIT (1:800, D151515, Sangon), p-PDGFRβ (1:800, D151409, Sangon); FXR1 (1:1000, BS70701, bioworld, China); YAP1 (1:1000, sc-398182, Santa Cruz, USA); T-STAT3 (1:1000, #4904, CST, USA), p-STAT3 (1:1000, #9131, CST), T-AKT (1:1000, #9272, CST), p-AKT (1:1000, #2965, CST), T-ERK (1:1000, #9102, CST), p-ERK (1:1000, #4348, CST), T-P38 (1:1000, #9212, CST), p-P38 (1:1000, #4631, CST) and β-Actin (1:1500, ZSBIO, China). For ELISA assay, conditioned medium was collected from 1 × 106 NFs or CAFs in a 6-well plate, and the concentrations of IL11 and IL15 were measured using a standard ELISA Kit (Raybiotech, USA) according to the manufacturer's instructions.
Vegfa
Manufactured by Wanlei
Sourced in China
VEGFA is a recombinant protein that functions as a growth factor. It is involved in the regulation of angiogenesis, the process of new blood vessel formation from existing vasculature.
Automatically generated - may contain errors
Lab products found in correlation
Ab53066, by Abcam (1 mentions)
Ab7213, by Abcam (1 mentions)
Ab89887, by Abcam (1 mentions)
Sc 398182, by Santa Cruz Biotechnology (1 mentions)
Wl00009b, by Wanlei (1 mentions)
Ab5694, by Abcam (1 mentions)
β actin, by ZSGB-BIO (1 mentions)
Ipvh00010, by Merck Group (1 mentions)
Gel pro analyzer, by Meyer Instruments (1 mentions)
Tgf β1, by Wanlei (1 mentions)
2 protocols using vegfa
1
Pathway analysis of fibroblast activation
2
Protein Expression Analysis by Western Blot
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Total protein was extracted using a standard procedure [30 (link)], separated on an 8–12% polyacrylamide gel (SDS-PAGE) and then transferred to a PVDF membrane (No. IPVH00010, Millipore, USA). vascular endothelial growth factor-A (VEGF-A, 1:500, No. WL03335, Wanleibio, Beijing, China), hypoxia inducible factor-1α (HIF-1α, 1:500, No. WL01607, Wanleibio, Beijing, China), transforming growth factor-β1 (TGF-β1; 1:500, No. WL02998, Wanleibio, Beijing, China) and β-actin (1:100, No. WL01372, Wanleibio, Beijing, China) were used as primary antibodies. Secondary antibodies were used at a 1:10,000 dilution, and protein expression was detected using an ECL assay (No. WLA003, Wanleibio, Beijing, China). Densitometry was conducted using the Gel Image Processing System (Gel-Pro Analyzer, Meyer Instruments, Houston, TX, USA). The immunoblots shown are representative of at least three independent experiments.
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