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2 protocols using mouse anti mavs

1

Antibodies for RIG-I Signaling Pathway

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Rabbit antibodies against RIG-1, MDA5, IRF3, phospho-IRF3, TBK1, phospho-TBK1, and TRIM25, were obtained from Cell Signaling Technology (Leiden, Netherlands). Rabbit antibody anti-RNF135 (RIPLET) was purchased from Sigma-Aldrich (MO, USA). Mouse anti-MAVS, anti-Sec13, and b-actin antibodies were purchased from Santa Cruz Biotechnology (TX, USA). Rabbit polyclonal anti-E5 serum recognising the C-terminal 14 amino acids of the BPV E5 oncoprotein was kindly gifted provided by Prof. DiMaio (Yale University, New Haven USA).
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2

Antibody-based protein detection protocol

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Protein A/G beads were purchased from Santa Cruz Biotechnology (USA), and the anti-Flag magnetic beads were purchased from Bimake. 2'3'-cGAMP was purchased from Invivogen. Rabbit anti-Myc-tag (71D10), rabbit anti-IRF3 (D83B9), rabbit anti-pIRF3 (4D46), rabbit anti-TBK1 (3031S), rabbit anti-pTBK1 (D52C2) were purchased from Cell Signaling Technology; Mouse anti-MAVS was purchased from Santa Cruz; Mouse anti-actin, mouse anti-V5-tag, and rabbit anti-calnexin were purchased from proteintech; Mouse anti-Flag M2 was purchased from Sigma Aldrich; Mouse anti-Myc-tag (9E10) was purchased from Origene; Rabbit anti-GM130 was purchased from Abcam; Rabbit anti-Tom20 antibody was purchased from Abclonal; Mouse anti-HA was purchased from MDL biotech.
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