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Anti human cd184 cxcr4 pe

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Anti-human CD184(CXCR4)-PE is a flow cytometry antibody reagent used for the detection and analysis of CD184 (CXCR4), a cell surface chemokine receptor, on human cells. The antibody is conjugated to the fluorescent dye phycoerythrin (PE) for visualization and quantification of CD184-expressing cells by flow cytometry.

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2 protocols using anti human cd184 cxcr4 pe

1

Quantifying Endoderm Induction and Hepatocyte Markers

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Endoderm induction was quantified using anti-human CD184(CXCR4)-PE (StemCell Technologies) and anti-human CD117(CKIT)-APC (ThermoFisher Scientific) conjugated monoclonal antibodies.21 (link),25 (link) To quantify intracellular protein content, iHeps were fixed in 1.6% paraformaldehyde for 20 min at 37°C and then permeabilized in saponin buffer (BioLegend). Cells were first probed using AAT (Santa Cruz Biotechnologies), AFP (Abcam), ZAAT (a kind gift from Qiushi Tang and Chris Mueller), and then anti-mouse IgG1-AlexaFluor647 (Jackson ImmunoResearch), anti-rabbit IgG-AlexaFluor488 (ThermoFisher Scientific) and anti-mouse IgG-AlexaFluor488 (ThermoFisher Scientific) antibodies. Staining quantification was performed using BD FACSCalibur or Stratedigm S1000EXi and all gating was performed using isotype-stained controls. Data analysis was performed using FlowJo (Tree Star) and Prism8 (GraphPad) software.
Kaserman J.E., Werder R.B., Wang F., Matte T., Higgins M.I., Dodge M., Lindstrom-Vautrin J., Bawa P., Hinds A., Bullitt E., Caballero I.S., Shi X., Gerszten R.E., Brunetti-Pierri N., Liesa M., Villacorta-Martin C., Hollenberg A.N., Kotton D.N, & Wilson A.A. (2022). Human iPSC-hepatocyte modeling of alpha-1 antitrypsin heterozygosity reveals metabolic dysregulation and cellular heterogeneity. Cell reports, 41(10), 111775.
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2

Quantifying Endoderm Induction and Hepatocyte Markers

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Endoderm induction was quantified using anti-human CD184(CXCR4)-PE (StemCell Technologies) and anti-human CD117(CKIT)-APC (ThermoFisher Scientific) conjugated monoclonal antibodies.21 (link),25 (link) To quantify intracellular protein content, iHeps were fixed in 1.6% paraformaldehyde for 20 min at 37°C and then permeabilized in saponin buffer (BioLegend). Cells were first probed using AAT (Santa Cruz Biotechnologies), AFP (Abcam), ZAAT (a kind gift from Qiushi Tang and Chris Mueller), and then anti-mouse IgG1-AlexaFluor647 (Jackson ImmunoResearch), anti-rabbit IgG-AlexaFluor488 (ThermoFisher Scientific) and anti-mouse IgG-AlexaFluor488 (ThermoFisher Scientific) antibodies. Staining quantification was performed using BD FACSCalibur or Stratedigm S1000EXi and all gating was performed using isotype-stained controls. Data analysis was performed using FlowJo (Tree Star) and Prism8 (GraphPad) software.
Kaserman J.E., Werder R.B., Wang F., Matte T., Higgins M.I., Dodge M., Lindstrom-Vautrin J., Bawa P., Hinds A., Bullitt E., Caballero I.S., Shi X., Gerszten R.E., Brunetti-Pierri N., Liesa M., Villacorta-Martin C., Hollenberg A.N., Kotton D.N, & Wilson A.A. (2022). Human iPSC-hepatocyte modeling of alpha-1 antitrypsin heterozygosity reveals metabolic dysregulation and cellular heterogeneity. Cell reports, 41(10), 111775.
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