A modified protocol was used for detection of expression of HIF1 and HIF2. For antigen retrieval, sections were immersed in preheated Dako target retrieval solution in a pressure cooker for 90 s. Primary antibodies were: mouse monoclonal anti-human HIF1α H1α67 (Novus Biologicals, Littleton, CO) 1/4000; rabbit polyclonal anti-mouse HIF2α PM8 antiserum (Novus Biologicals, Littleton, CO), 1/100. Primary antibody was omitted for negative controls. Antigen/antibody complexes were revealed by means of the Catalyzed Signal Amplification system (DAKO, Carpinteria, CA, Dako) (HIF) according to the manufacturer’s instructions.
Anti nse
Anti-NSE is a laboratory reagent used for the detection of neuron-specific enolase (NSE) in biological samples. NSE is a glycolytic enzyme found predominantly in neurons and neuroendocrine cells, and its levels can be used as a biomarker for certain neurological conditions. The Anti-NSE reagent provides a reliable method for quantifying NSE levels in research and diagnostic applications.
Lab products found in correlation
3 protocols using anti nse
Immunohistochemical Detection of CD31, NSE, HIF1, and HIF2
Quantifying Protein Expression via Western Blot
Lineage Tracing of CgA-Expressing Cells
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!