Fetal bovine serum albumin fbs

Itraconazole (I129771) was purchased from Aladdin. IR820 (448014) was obtained from J&K Scientific. 3-methyladenine (3-MA) was purchased from MedChem Express. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dimethyl sulfoxide, crystal violet (C0775), and chloroquine (CQ) diphosphate salt (C6628) were purchased from Millipore Sigma. DAPI was purchased from Invitrogen. Sodium chloride injection was purchased from Sichuan Kelun Pharmaceutical Co., Ltd. Mel (Purity, 99.5%). Penicillin-streptomycin solution was manufactured for HyClone Laboratories. Fetal bovine serum albumin (FBS) was purchased from Biowest. Dulbecco's modified eagle medium (DMEM) was purchased from Thermo Fisher Biochemical Products (Beijing) Co., Ltd. The antibodies used were as follows: anti-β-actin was purchased from Santa Cruz Biotechnology; cleaved-caspase 3 caspase 3, p62, PARP, cleaved-PARP were acquired from Cell Signaling Technology; Ki67 was purchased from Bioss; and LC3 was purchased from Novus. Horseradish peroxidase-conjugated anti-rabbit secondary antibody was obtained from Santa Cruz Biotechnology. For immunofluorescence, goat anti-rabbit Alexa Fluor 488 and goat anti-mouse Alexa Fluor 594 were obtained from Thermo Fisher Scientific.

Thymopentin (TP5) was purchased from Meilunbio. Doxorubicin hydrochloride (DOX) was obtained from Aladdin. Fetal bovine serum albumin (FBS) was purchased from Biowest. Dulbecco's modified Eagle's medium (DMEM) was purchased from Gibco. All assay kits, if not specified, were acquired from Beyotime. The antibodies used were as follows: anti-calreticulin, HMGB1, and CD8 were purchased from Santa Cruz Biotechnology; Ki67 was purchased from Bioss; anti-mouse antibodies (FITC-labeled CD3, FITC-labeled CD11c, FITC-labeled CD25, PE-labeled CD8a, PE-labeled CD86, APC-labeled CD4, APC-labeled CD80, Alexa Flour 647-labeled Foxp3) were all purchased from BioLegend. D-Luciferin, Potassium Salt was purchased from Yeasen.