Fluorescein isothiocyanate (fitc)

Manufactured by Biotium

Sourced in Singapore

FITC is a fluorescent dye commonly used in biological research. It is a highly fluorescent molecule that emits green light when excited by a blue light source. FITC can be used to label proteins, cells, and other biomolecules for detection and analysis purposes.

Automatically generated - may contain errors

Lab products found in correlation

Cyclophosphamide, by MedChemExpress (1 mentions) Fm4 64fx, by Thermo Fisher Scientific (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Thermo Fisher Scientific (1 mentions) N 3 dimethylaminopropyl n ethylcarbodiimide hydrochloride edc hcl, by GL Biochem (1 mentions) 1 hydroxybenzotriazole hobt, by GL Biochem (1 mentions)

3 protocols using fluorescein isothiocyanate (fitc)

Synthesis and Characterization of Novel Biomaterials

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All chemicals used in the syntheses unless otherwise specified were purchased from Sigma-Aldrich Co. LLC. l-lysine and DiS-C3-(5) were purchased from Combi-Blocks, Inc. N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC.HCl) and 1-hydroxybenzotriazole (HOBt) were purchased from GL Biochem Ltd. Cyclophosphamide was purchased from MedChemExpress LLC. PI and FM 4–64FX were purchased from Thermo Fisher Scientific. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Alfa Aesar. FITC was purchased from Biotium, Inc. Technical grade solvents were purchased from SG Labware Pte Ltd. (Singapore) and directly used for column purification without any distillation. Pullulan standards were purchased from Polymer Standards Service.

Zhong W., Shi Z., Mahadevegowda S.H., Liu B., Zhang K., Koh C.H., Ruan L., Chen Y., Zeden M.S., Pee C.J., Marimuthu K., De P.P., Ng O.T., Zhu Y., Chi Y.R., Hammond P.T., Yang L., Gan Y.H., Pethe K., Greenberg E.P., Gründling A, & Chan-Park M.B. (2020). Designer broad-spectrum polyimidazolium antibiotics. Proceedings of the National Academy of Sciences of the United States of America, 117(49), 31376-31385.

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Affinity-based Cellular Interactome Analysis

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After final round of selection against chaperones or WT cell extract, enriched populations were collected and incubated both with chaperones or WT cell extract and Ugi. Chaperones and WT cell extracts were labeled with APC (Biotium # 92108) while Ugi was labeled with FITC (Biotium # 92103) as described by the manufacturer. Co-incubated populations were analyzed by Bio-Rad

Melanker O., Goloubinoff P, & Schreiber G. (2022). In vitro Evolution of Uracil Glycosylase Towards DnaKJ and GroEL Binding Evolves Different Misfolded States. Journal of molecular biology, 434(13).

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Affinity-based Cellular Interactome Analysis

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Melanker O., Goloubinoff P., & Schreiber G. (2021). In vitro evolution of uracil glycosylase towards DnaKJ and GroEL binding evolves different misfolded states.

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