Streptavidin alp

Manufactured by Merck Group

Streptavidin ALP is a laboratory reagent used in various immunoassay and molecular biology techniques. It is a conjugate of streptavidin, a protein derived from the bacterium Streptomyces avidinii, and alkaline phosphatase (ALP), an enzyme. The primary function of Streptavidin ALP is to facilitate the detection and quantification of biotinylated molecules, such as proteins, nucleic acids, or other biomolecules, through a colorimetric or chemiluminescent reaction.

Automatically generated - may contain errors

Lab products found in correlation

Bcip nbt phosphatase substrate, by Merck Group (1 mentions) B1140, by Merck Group (1 mentions) Msipn4510, by Merck Group (1 mentions)

2 protocols using streptavidin alp

STIP1-Specific T Cell Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Multiscreen_HTS filter Elispot plates (Mabtech, Stockholm, Sweden) were coated with 15 μg/mL of human IFNγ antibody (1-DIK) overnight at 4 °C. A total of 100,000 PBMCs were placed in each well and stimulated with 5 μg/mL of pooled and/or individual STIP1 peptides from the aforementioned peptide library for 18 h with 5 IU/mL of IL-2. The CD3/CD28/CD2 T cell activator (STEMCELL Technologies, Vancouver, BC, Canada) was used as the positive control. The plates were then developed using the 1:1000 human biotinylated IFNγ detection antibody (7-B6–1), followed by streptavidin ALP and BCIP/NBT phosphatase substrate (Sigma Aldrich). The number of spot forming units (SFU) were quantified using the Mabtech Iris^TM FluoroSpot/ELISpot reader system equipped with Spot reader software version 1.1.9 and included in the analysis. Out of the 15 individuals with high STIP1 autoantibodies, seven individuals returned for the follow-up and were recruited for the characterisation of STIP-specific T cells.

Tan J.S., Lee B., Lim J., Ma D.R., Goh J.X., Goh S.Y., Gulam M.Y., Koh S.M., Lee W.W., Feng L., Wang Q., Chao Y., Rötzschke O, & Tan E.K. (2022). Parkinson’s Disease-Specific Autoantibodies against the Neuroprotective Co-Chaperone STIP1. Cells, 11(10), 1649.

+ Open protocol

+ Expand

DENV-specific Antibody Secreting Cell Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Enzyme-linked immunospot plates (MSIPN4510; Millipore) were coated with either antibody to human immunoglobulin (H17000; Caltag) or ultraviolet irradiation–inactivated DENV-1–DENV-4. Plates were washed with RPMI medium and nonspecific binding was blocked by incubation for 1 h with 1% BSA in RPMI medium. Sorted antibody-secreting cells were added at 500 cells in wells coated with anti-immunoglobulin and DENV and samples were incubated overnight at 37 °C in 5% CO₂. Plates were washed and then were incubated for 2 h at room temperature with biotinylated antibody to human IgG (B1140; Sigma) or IgM (B1265; Sigma), followed by streptavidin-ALP (S2890; Sigma). Reactions were developed and spots were counted with an AID Elispot plate reader.

Dejnirattisai W., Wongwiwat W., Supasa S., Zhang X., Dai X., Rouvinski A., Jumnainsong A., Edwards C., Quyen N.T., Duangchinda T., Grimes J.M., Tsai W.Y., Lai C.Y., Wang W.K., Malasit P., Farrar J., Simmons C.P., Zhou Z.H., Rey F.A., Mongkolsapaya J, & Screaton G.R. (2014). A new class of highly potent, broadly neutralizing antibodies isolated from viremic patients infected with dengue virus. Nature immunology, 16(2), 170-177.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!