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Hmec cells

Manufactured by Lonza
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HMEC cells are a type of primary human mammary epithelial cells derived from normal human mammary tissue. These cells are commonly used in cell culture studies related to breast biology and cancer research.

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Lab products found in correlation

Magna chip g chromatin immunoprecipitation kit, by Merck Group (2 mentions) H 129, by Santa Cruz Biotechnology (2 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Fungizone, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Mcf 7, by American Type Culture Collection (1 mentions) Hcc1806, by American Type Culture Collection (1 mentions) Mda mb 468, by American Type Culture Collection (1 mentions) Du4475, by American Type Culture Collection (1 mentions) Mda mb 157, by American Type Culture Collection (1 mentions) Hcc1937, by American Type Culture Collection (1 mentions) Hcc70, by American Type Culture Collection (1 mentions) Hs578t, by American Type Culture Collection (1 mentions) Au565, by American Type Culture Collection (1 mentions) Zr 75 30, by American Type Culture Collection (1 mentions) Cama 1, by American Type Culture Collection (1 mentions) Hcc1500, by American Type Culture Collection (1 mentions) Hcc1569, by American Type Culture Collection (1 mentions) Hcc202, by American Type Culture Collection (1 mentions) Uacc 3199, by American Type Culture Collection (1 mentions)

Close spelling variants of this product

HMEC human mammary epithelial cells and derivatives HMECs

8 protocols using hmec cells

1

Culturing Human Mammary and Breast Cancer Cells

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Human mammary epithelial (HMEC) cells were purchased from Lonza (Basel, Switzerland) and breast carcinoma cell lines 184A1, AU-565, CAMA-1, DU-4475, HCC-1500, HCC-1569, HCC-1806, HCC-1937, HCC-202, HCC-70, Hs578T, MCF-7, MDA-MB-157, MDA-MB-175V11, MDA-MB-468, T-47D, UACC-3199 and ZR-75-30 were purchased from American Type Culture Collection (Manassas, VA). Cells were tested negative for mycoplasma contamination and validated for species and unique DNA profile using short tandem repeat analysis by the provider or by the authors. All cell lines were cultured in RPMI Medium 1640 (Life Technologies, Carlsbad, CA) supplemented with 10% fetal bovine serum, 1% Antibiotic-Antimycotic containing penicillin, streptomycin and Fungizone (Invitrogen, Carlsbad, CA), and 1% HEPES at 37 °C in an atmosphere containing 5% CO2.
Han Y.J., Boatman S.M., Zhang J., Du X.C., Yeh A.C., Zheng Y., Mueller J, & Olopade O.I. (2018). LncRNA BLAT1 is Upregulated in Basal-like Breast Cancer through Epigenetic Modifications. Scientific Reports, 8, 15572.
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2

Cell Culture Conditions for Breast Epithelial Lines

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HMLE cells were cultured in DMEM/F12 (1:1) media containing 5% horse serum, 10μg/ml insulin, 20 ng/nl EGF, 100ng/ml cholera toxin and 500ng/ml Hydrocortizone. iMMEC cells were cultured in F12 media containing 10% fetal bovine serum, 10μg/ml insulin, 20 ng/nl EGF and 500ng/ml Hydrocortizone. HMEC cells (Lonza) were cultured in media in accordance with manufacturer’s instructions.
Chakrabarti R., Wei Y., Hwang J., Hang X., Blanco M.A., Choudhury A., Tiede B., Romano R.A., DeCoste C., Mercatali L., Ibrahim T., Amadori D., Kannan N., Eaves C.J., Sinha S, & Kang Y. (2014). ΔNp63 promotes stem cell activity in mammary gland development and basal-like breast cancer by enhancing Fzd7 expression and Wnt signaling. Nature cell biology, 16(10), 1004-13.
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3

Virus-based Breast Cancer Cell Lines

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Construction of MV-m-uPA and MV-h-uPA, virus rescue, propagation and titration were performed as previously reported [24 (link), 36 (link)]. 4T1 cells (murine mammary carcinoma), MDA-MB-231 cells (human breast cancer), MCF-7 cells (human breast cancer), MDA-MB-436 cells (human breast cancer) were purchased from the American Type Culture Collection (ATCC, Manassas, VA). 4T1-luc2 cells stably expressing highly efficient luciferase were from PerkinElmer (Santa Clara CA). Cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS), penicillin and streptomycin at 37 °C and 5% CO2. HMEC cells (human mammary epithelial cells) were purchased from Lonza (Walkersville, MD) and maintained in MEGM according to manufacture’s instruction at 37 °C and 5% CO2. NMuMG (murine mammary epithelial cells) were purchased from the ATCC and maintained in DMEM containing 10% fetal bovine serum (FBS), penicillin and streptomycin at 37 °C and 5% CO2. Vero-αHis cells [36 (link)] were grown in DMEM containing 10% FBS at 37 °C and 5% CO2.
Jing Y., Bejarano M.T., Zaias J, & Merchan J.R. (2014). In vivo antimetastatic effects of uPAR retargeted measles virus in syngeneic and xenograft models of mammary cancer. Breast cancer research and treatment, 149(1), 99-108.
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4

ChIP-qPCR analysis of FZD7 regulation

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HMEC cells (Lonza) or MMTV-Wnt-1 primary cells were grown to 80% confluence and cells were then cross-linked with 1% formaledyde and processed. The crosslinking, immunoprecipitation, washing, elution, reverse crosslinking, and proteinase K treatment were performed according to the manufacturer’s directions described in the Magna ChIP G Chromatin Immunoprecipitation Kit from Millipore. Antibodies used were anti-4A4, H-129 (Santa Cruz) or normal rabbit IgG. Purified immunoprecipitated DNA was used for real time qPCR. Primers for ChIP PCR are: 5′-TATCAGCATTCCAGGCCCAC-3′ (forward) and 5′-TTCCTGGGAGAACAATCGCC-3′ (reverse) for human FZD7; 5′-AATGAGGCAAACACCCCCTC-3′ (forward) and 5′-CTCCGGGGGATTAAAGGTGG-3′ (reverse) for mouse Fzd7; 5′-CGAGATCCCTCCAAAATCAA (forward) and 5′-CCCAGCCTTCTCCATGG (reverse) for human GAPDH and 5′-AACATCAAATGGGGTGAGG-3′ (forward), 5′-GGCCTTCTCCATGGTGGT-3′ (reverse) for mouse Gapdh.
Chakrabarti R., Wei Y., Hwang J., Hang X., Blanco M.A., Choudhury A., Tiede B., Romano R.A., DeCoste C., Mercatali L., Ibrahim T., Amadori D., Kannan N., Eaves C.J., Sinha S, & Kang Y. (2014). ΔNp63 promotes stem cell activity in mammary gland development and basal-like breast cancer by enhancing Fzd7 expression and Wnt signaling. Nature cell biology, 16(10), 1004-13.
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5

Culturing HMEC and MDAMB231 Cells

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HMEC cells were obtained from Lonza (Lonza, Walkersville, MD) and cultured under recommended conditions. MDAMB231 cells were obtained from American Type Culture Collection (ATCC, Manassas, VA). MDAMB231 cells were cultured in DMEM with 5% FBS.
Rhie S.K., Hazelett D.J., Coetzee S.G., Yan C., Noushmehr H, & Coetzee G.A. (2014). Nucleosome positioning and histone modifications define relationships between regulatory elements and nearby gene expression in breast epithelial cells. BMC Genomics, 15(1), 331.
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6

Cell Culture Maintenance Protocol

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HEK
293T, HFF and A549 cell lines were
obtained from American Tissue Culture Collection (ATCC, Manassas,
VA). MDA-MB-231, MDA-MB-468, and MCF-7 cell lines were obtained from
Developmental Therapeutics Program at the National Cancer Institute
(NCI). The cells were maintained in DMEM (Life Technologies, Grand
Island, NY) with nonessential amino acids (Life Technologies) and
10% (v/v) HyClone fetal bovine serum (FBS, GE Healthcare Life Science,
Logan, UT) at 37 °C with 5% CO2. HMEC cells were obtained
from Lonza (Walkersville, MA) and were cultured in MEGM complete media
(Lonza) supplemented with 10 μg/mL penicillin and 10 μg/mL
streptomycin (Life Technologies) at 37 °C under 5% CO2.
Xie F., Li B.X., Kassenbrock A., Xue C., Wang X., Qian D.Z., Sears R.C, & Xiao X. (2015). Identification of a Potent Inhibitor of CREB-Mediated Gene Transcription with Efficacious in Vivo Anticancer Activity. Journal of Medicinal Chemistry, 58(12), 5075-5087.
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7

Cell Culture Conditions for Breast Epithelial Lines

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HMLE cells were cultured in DMEM/F12 (1:1) media containing 5% horse serum, 10μg/ml insulin, 20 ng/nl EGF, 100ng/ml cholera toxin and 500ng/ml Hydrocortizone. iMMEC cells were cultured in F12 media containing 10% fetal bovine serum, 10μg/ml insulin, 20 ng/nl EGF and 500ng/ml Hydrocortizone. HMEC cells (Lonza) were cultured in media in accordance with manufacturer’s instructions.
Chakrabarti R., Wei Y., Hwang J., Hang X., Blanco M.A., Choudhury A., Tiede B., Romano R.A., DeCoste C., Mercatali L., Ibrahim T., Amadori D., Kannan N., Eaves C.J., Sinha S, & Kang Y. (2014). ΔNp63 promotes stem cell activity in mammary gland development and basal-like breast cancer by enhancing Fzd7 expression and Wnt signaling. Nature cell biology, 16(10), 1004-13.
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8

ChIP-qPCR analysis of FZD7 regulation

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HMEC cells (Lonza) or MMTV-Wnt-1 primary cells were grown to 80% confluence and cells were then cross-linked with 1% formaledyde and processed. The crosslinking, immunoprecipitation, washing, elution, reverse crosslinking, and proteinase K treatment were performed according to the manufacturer’s directions described in the Magna ChIP G Chromatin Immunoprecipitation Kit from Millipore. Antibodies used were anti-4A4, H-129 (Santa Cruz) or normal rabbit IgG. Purified immunoprecipitated DNA was used for real time qPCR. Primers for ChIP PCR are: 5′-TATCAGCATTCCAGGCCCAC-3′ (forward) and 5′-TTCCTGGGAGAACAATCGCC-3′ (reverse) for human FZD7; 5′-AATGAGGCAAACACCCCCTC-3′ (forward) and 5′-CTCCGGGGGATTAAAGGTGG-3′ (reverse) for mouse Fzd7; 5′-CGAGATCCCTCCAAAATCAA (forward) and 5′-CCCAGCCTTCTCCATGG (reverse) for human GAPDH and 5′-AACATCAAATGGGGTGAGG-3′ (forward), 5′-GGCCTTCTCCATGGTGGT-3′ (reverse) for mouse Gapdh.
Chakrabarti R., Wei Y., Hwang J., Hang X., Blanco M.A., Choudhury A., Tiede B., Romano R.A., DeCoste C., Mercatali L., Ibrahim T., Amadori D., Kannan N., Eaves C.J., Sinha S, & Kang Y. (2014). ΔNp63 promotes stem cell activity in mammary gland development and basal-like breast cancer by enhancing Fzd7 expression and Wnt signaling. Nature cell biology, 16(10), 1004-13.
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