Anakinra

Manufactured by MedChemExpress

Sourced in United States

Anakinra is a laboratory equipment product manufactured by MedChemExpress. It is used for the detection and quantification of the interleukin-1 receptor antagonist (IL-1Ra) protein.

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Lab products found in correlation

Tak 242, by Merck Group (1 mentions) Tlr3 dsrna complex inhibitor, by Merck Group (1 mentions) Mcc950, by MedChemExpress (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Thermo Fisher Scientific (1 mentions) Mitosox, by Thermo Fisher Scientific (1 mentions) Gm csf, by Thermo Fisher Scientific (1 mentions) M csf, by Thermo Fisher Scientific (1 mentions) N acetyl cysteine (nac), by Merck Group (1 mentions) A205804, by Santa Cruz Biotechnology (1 mentions) Phorbol myristate acetate (pma), by Cayman Chemical (1 mentions) Axitinib, by Pfizer (1 mentions) Atezolizumab, by Roche (1 mentions) Cabozantinib, by Ipsen (1 mentions)

4 protocols using anakinra

Investigating Anakinra's Role in LPS-Induced Hearing Loss

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To evaluate the roles for anakinra in LPS-induced EH, mice were intraperitoneal injected (i.p.) with anakinra (10 mg/kg, Med Chem Express, Monmouth Junction, NJ, USA) or equivalent NS 30 min before LPS challenge. In all cases, hearing and vestibular function were tested on day 5 after first dose of LPS injection. After then, those mice were anesthetized by intraperitoneal injection with a mixture of xylazine (10 mg/kg) and ketamine (100 mg/kg) and inner ears were subjected to protein or RNA expression and EH analysis.

Zhang N., Li N., Wang S., Xu W., Liu J., Lyu Y., Li X., Song Y., Kong L., Liu Y., Guo J., Fan Z., Zhang D, & Wang H. (2022). Protective effect of anakinra on audiovestibular function in a murine model of endolymphatic hydrops. Frontiers in Cellular Neuroscience, 16, 1088099.

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Inhibition of SARS-CoV-2 Cellular Entry

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The cells were pre-incubated with anakinra (an IL-1R inhibitor; MedChemExpress, South Brunswick, NJ, USA), TLR3/dsRNA complex inhibitor (Merck Millipore, Germany), TAK-242 (a TLR4 inhibitor; Merck Millipore, Germany), and infliximab (an anti-TNF-α monoclonal antibody; MedChemExpress) for 30 min. The cells were then treated with SARS-CoV-2 solutions at various dilution ratios and cultured for 48 or 72 h in the presence of each inhibitor. The cytotoxicity of the inhibitors was assessed using the Cell Counting Kit8 in a concentration-dependent manner, and the optimal dosage was determined.

Nakazawa D., Takeda Y., Kanda M., Tomaru U., Ogawa H., Kudo T., Shiratori-Aso S., Watanabe-Kusunoki K., Ueda Y., Miyoshi A., Hattanda F., Nishio S., Uozumi R., Ishizu A, & Atsumi T. (2023). Inhibition of Toll-like receptor 4 and Interleukin-1 receptor prevent SARS-CoV-2 mediated kidney injury. Cell Death Discovery, 9, 293.

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Targeted miRNA Modulation in Mice

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MCC950 and anakinra were purchased from MedChemExpress (Princeton, NJ, USA) and administered intraperitoneally at 40 mg/kg/day to mice. miR-223 was administered as the synthesized mmu-miR-223 via a nanoparticle. MaxSuppressor In Vivo RNA-LANCEr II (NLE) (Bioscientific, Austin, TX, USA) was used according to the manufacturer’s instructions to administer 100 μg/kg/day mmu-miR-22 mimics intravenously.

Feng Z., Qi S., Zhang Y., Qi Z., Yan L., Zhou J., He F., Li Q., Yang Y., Chen Q., Xiao S., Li Q., Chen Y, & Zhang Y. (2017). Ly6G+ neutrophil-derived miR-223 inhibits the NLRP3 inflammasome in mitochondrial DAMP-induced acute lung injury. Cell Death & Disease, 8(11), e3170-.

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Comprehensive Reagent Acquisition for Biological Assays

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PMA (catalog 10008014) was purchased from Cayman Chemical. NAC (catalog A7250) and cis-diammineplatinum(II) dichloride (catalog 479306) were purchased from MilliporeSigma. MTT (catalog M6494), DCF (catalog C6827), and MitoSOX (catalog M36008) were purchased from Invitrogen. The ICAM1 inhibitor, A205804 (catalog sc-203484), was purchased from Santa Cruz Biotechnology. The IL-1R antagonist, anakinra (catalog HY-108841), was purchased from MedChemExpress. G M-CSF and M-CSF were purchased from PeproTech. GRO-α, IL-1α, IL-1β, IL-18, MIP-1β, SDS-1α, and VEGF were purchased from CROYEZ. Neutralizing antibodies against human IL-1β (4H5) and isotype-matched IgG (T8E5) were purchased from InvivoGen. ELISA kits for human IL-1β (catalog ARG80101) and mouse IL-1β (catalog ARG80196) were purchased from Arigo Biolaboratories. Clinical medications used in the present study and corresponding suppliers were DTX (Enzo), axitinib (Pfizer), PIO (Takeda), atezolizumab (Roche), cabozantinib (Ipsen), and Asadin (TTY Biopharm).

Hsieh C.Y., Lin C.C., Huang Y.W., Chen J.H., Tsou Y.A., Chang L.C., Fan C.C., Lin C.Y, & Chang W.C. (2022). Macrophage secretory IL-1β promotes docetaxel resistance in head and neck squamous carcinoma via SOD2/CAT-ICAM1 signaling. JCI Insight, 7(23), e157285.

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