Oasis hlb 200 mg cartridge

Manufactured by Waters Corporation

Sourced in United States

The Oasis HLB (200 mg) cartridges are solid-phase extraction (SPE) products designed for sample preparation and cleanup. They utilize hydrophilic-lipophilic balance (HLB) sorbent, which is effective for extraction of a wide range of polar and non-polar analytes from various sample matrices.

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Lab products found in correlation

Qtrap 5500, by AB Sciex (1 mentions) Acquity uhplc system, by Waters Corporation (1 mentions) Itraq reagent 8 plex kit, by AB Sciex (1 mentions) Sequence grade modified trypsin, by Promega (1 mentions) Trypsin, by Promega (1 mentions)

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Oasis HLB cartridges (276 citations) Oasis HLB (164 citations) HLB cartridges (38 citations) Oasis cartridges (17 citations) Oasis HLB 200 mg (4 citations) Oasis HLB 200 mg extraction cartridges (2 citations)

4 protocols using oasis hlb 200 mg cartridge

Automated SPE and UHPLC-MS/MS Analysis

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Rationale for the selection of these contaminants is explained in Supplementary information (SI paragraph 2).
Organic contaminants were extracted from 1 L water using automated solid-phase extraction (SPE) with Oasis HLB (200 mg) cartridges (Waters Corporation, Milford, MA, USA) using an Autotrace AT280 SPE workstation (Thermo Scientific, Waltham, MA, USA). Analyses were performed by ultra-high pressure liquid chromatography tandem mass spectrometry (UHPLC-MS-MS), which was performed with an Acquity® UHPLC system (Waters) coupled to a hybrid triple-quadrupole linear ion trap mass spectrometer 5500 QTRAP® with a turbo ion spray source from AB SCIEX (Foster City, CA, USA). Experimental details are given in Loos et al. (2017) (link). The internal surrogate standards used for “isotope dilution” quantification are depicted in Table SI5. The Limits of Quantification (LOQs) for the target analytes are shown in Table SI6.

Saccà M.L., Ferrero V.E., Loos R., Di Lenola M., Tavazzi S., Grenni P., Ademollo N., Patrolecco L., Huggett J., Caracciolo A.B, & Lettieri T. (2019). Chemical mixtures and fluorescence in situ hybridization analysis of natural microbial community in the Tiber river. The Science of the Total Environment, 673, 7-19.

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Sampling of Indoor Air Volatiles

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Solvents, standards and sorbents OASIS HLB (200 mg) cartridges were purchased from Waters. This polymer was employed for the concentration of condensed water, and also as trapping sorbent for active air sampling. Dehumidifiers employed to obtain water samples from different indoor areas were purchased in local markets. They were furnished with a cryogenic fluid evaporator where water vapor condensates. A grid in the back side of these appliances served as a filter to limit the entrance of coarse particles into the evaporator unit. In some experiments, the grid was covered with glass fibre filters (GFF), pore size of 0.7 µm, provided by Sigma-Aldrich.
The membrane pump employed for active sampling of indoor air was purchased from Vaccubrand (model MD 4NT). It was connected to an HLB cartridge, followed by a rotameter. Air was sampled at a flow rate of 16.5 L min -1 , similar to values considered in previous studies dealing with determination of fragrances (10 L min -1 ) (Lamas et al., 2010) (link) and other semi-volatile compounds from indoor air (12 L min -1 ) (Laboire et al., 2016) (link). Unless otherwise stated, the pump was operated 5 h to concentrate 5 m 3 of air.

Cobo-Golpe M., Ramil M., Cela R, & Rodríguez I. (2020). Portable dehumidifiers condensed water: A novel matrix for the screening of semi-volatile compounds in indoor air. Chemosphere, 251.

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iTRAQ Labeling and Analysis of Mutant Samples

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4 control samples and 7 mutant samples were analyzed in two separate iTRAQ experiments. 40 μg of each control sample was taken and pooled together to make a combined reference sample. This reference sample was labeled with iTRAQ reagent 113 and included in both LC-MS/MS runs, which allows for accurate comparisons across multiple LC-MS/MS runs. iTRAQ labeling was carried out using iTRAQ Reagent 8-Plex kit (AB Sciex, Foster City, CA) and followed the manufacturer's protocol with minor modifications. For each sample, 40 μg protein was used, and the concentration was adjusted to 1 μg/μl with iTRAQ dissolution buffer, reduced with 2 μl Reducing Reagent (TCEP solution) for 60 min at 60°C, alkylated with 1 μl Cysteine Blocking Reagent (MMTS solution) at RT for 20 min, and digested overnight with sequence grade modified trypsin (Promega, Madison, WI) at 37°C with a trypsin to protein ratio of 1∶50 (w/w). Each iTRAQ 8-plex reagent was dissolved in 150 μl isopropanol, vortexed, and added to each sample. The sample/reagent mixture was incubated at room temperature for 3 hr. Labeled peptides were combined, and then desalted using an Oasis HLB 200 mg cartridge (Waters, Milford, MA).

Hauser D.N., Dillman A.A., Ding J., Li Y, & Cookson M.R. (2014). Post-Translational Decrease in Respiratory Chain Proteins in the Polg Mutator Mouse Brain. PLoS ONE, 9(4), e94646.

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Phosphopeptide Enrichment via Fe-IMAC

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Phosphopeptide enrichment was performed using Fe-IMAC as previously described (Swaney and Villén, 2016 ). Briefly, 3.3 mg of dried peptides were solubilized in 900 μL of phosphopeptide binding solution (80% acetonitrile and 0.1% TFA). 150 μL peptide aliquots were mixed with 165 μL of Fe-IMAC and incubated at room temperature for 30 minutes with shaking. The supernatant and all washes were collected, dried by speedvac, and desalted on an Oasis HLB 200 mg cartridge (Waters) to be later used for peptide fractionation and total protein quantification via nanoLC-MS/MS. The phosphopeptides were quickly eluted from beads with 100 μL of phosphopeptide elution solution (70% acetonitrile and 1% ammonium hydroxide), passed through C8 StageTip and acidified with 30 μL of 10% formic acid. The phosphopeptide eluents were dried by speedvac and desalted using SDB StageTips.

Abt E.R., Le T.M., Dann A.M., Capri J.R., Poddar S., Lok V., Li L., Liang K., Creech A.L., Rashid K., Kim W., Wu N., Cui J., Cho A., Lee H.R., Rosser E.W., Link J.M., Czernin J., Wu T.T., Damoiseaux R., Dawson D.W., Donahue T.R, & Radu C.G. (2022). Reprogramming of nucleotide metabolism by interferon confers dependence on the replication stress response pathway in pancreatic cancer cells. Cell reports, 38(2), 110236.

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