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Szh stereomicroscope

Manufactured by Olympus
Sourced in Japan

The SZH stereomicroscope is a high-quality optical instrument designed for a variety of laboratory applications. It provides a stereoscopic, three-dimensional view of specimens, enabling users to observe and analyze samples in detail. The SZH features binocular eyepieces, a wide range of magnification options, and a sturdy construction for reliable performance.

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6 protocols using szh stereomicroscope

1

Imaging Preserved Termite Specimens

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Preserved workers, stored in 85% ethanol, were positioned in a transparent petri dish filled with Purell® hand sanitizer (70% EtOH). Body sections and dissected guts were photographed as multi-layer montages using a Leica M205C stereomicroscope with a Leica DFC 425 module run with Leica Application Suite software version 3. Mandibles and EVA were mounted on slides with PVA mounting medium (Bioquip Products, Inc.) and photographed with a Leica CTR 5500 compound microscope using bright field lighting and the same montage software. Imagos were photographed in alcohol on sand. Terminology of the worker gut follows that of Sands (1972) and Noirot (2001) (link). Measurements were obtained using an Olympus SZH stereomicroscope fitted with an ocular micrometer. All specimens described here are deposited in the authors’ collections under the accession numbers AFR1508 and AFR1282 for RHS and CGO060 for YR.
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2

Lotus Seed Structure Observation

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The structure of the lotus seeds was observed under an Olympus SZH Stereomicroscope (Olympus Co., Tokyo, Japan).
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3

Nematode Extraction and Morphological Characterization from Carrot Rhizosphere

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Soil samples were collected from the rhizosphere of carrot (Daucus carota L.) in Dezful, Khuzestan province, southwestern Iran. Nematodes were extracted using the tray method (Whitehead and Hemming, 1965 ), killed and fixed by adding hot FPG (4:1:1, formaldehyde: propionic acid: glycerin), then transferred to anhydrous glycerin following the method of De Grisse (1969). To prepare nematodes for morphological observations, fixed specimens of the new species were handpicked under a Olympus SZH stereo microscope, and mounted in a small drop of pure glycerin supported with paraffin on permanent glass slides. Morphological characters were examined using a Leitz Dialux 22 light microscope. Morphometric characters and photographs were taken using a Dino-Eye digital eyepiece camera (Model AM7023, bundled with the DinoCapture 2.0 software; AnMo Electronics Corporation; New Taipei City; Taiwan) adjoined to the aforementioned microscope. Line drawings were first made using a drawing tube attached to the microscope, then redrawn and prepared for publication using CorelDRAW® software version 16.
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4

Termite Species Distribution Mapping and Morphological Analysis

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The distribution map (Fig. 1) was created from sample localities in the University of Florida Termite Collection (UFTC) database (Scheffrahn 2019, Table S1) and localities from Banks 1920 (link), Light 1930a , Light 1930b , and Nickle & Collins 1988 . Measurements (Fig. 2) were obtained using an Olympus SZH stereomicroscope fitted with an ocular micrometer. Preserved soldiers (Figs 3-5), stored in 85% ethanol, were positioned in a transparent petri dish filled with Purell® hand sanitizer (70% EtOH) and photographed as multi-layer montages using a Leica M205C stereomicroscope with a Leica DFC 425 module run with Leica Application Suite software version 3. Enteric valve armatures (Fig. 5) were mounted on slides with PVA mounting medium (Bioquip Products, Inc.) and photographed with a Leica CTR 5500 compound microscope using bright field lighting and the same montage software.
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5

Stereomicroscopy imaging of preserved specimens

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Figures were taken as multi-layer montages using a Leica M205C stereomicroscope with a Leica DFC 425 module run with Leica Application Suite version 3 software. Preserved specimens were taken from 85% ethanol and suspended in a pool of Purell® hand sanitizer (70% EtOH) to position the specimens over a transparent plastic Petri dish background. Measurements (Tables 123) were obtained using an Olympus SZH stereomicroscope fitted with an ocular micrometer. The field photograph of live specimens, placed in a filter paper-lined Petri dish (Fig. 3), were taken with a Canon EOS 6D camera combined with Canon MP-E 65/2.8 macro lens.
Material examined.
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6

Millipede Tarsi Morphology Protocol

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Specimens were field-preserved in 90% isopropanol and later transferred to 70% ethanol. Morphological studies were done using an Olympus SZH stereomicroscope and an Olympus BX50 compound microscope equipped with Nomarski optics. Gonopods were temporarily mounted on microscope slides in glycerine for study up to 400X magnification and drawings were made from these slides using a drawing tube on the BX50. For scanning electron microscopy (SEM), specimens were mounted on 12.7 mm diameter aluminum stubs, using double-sided carbon discs. These were sputter coated with a 40 nm thick layer of platinum and palladium, using a Leica EM ACE600 high vacuum sputter coater. SEM micrographs were taken with a FEI Quanta 600 FEG environmental scanning electron microscope. Photographs were edited and refined using GIMP, and plates were composed in InkScape.
Type specimens are deposited in the collection of the California Academy of Sciences, San Francisco, California, USA, along with the SEM stub, WS36-15, to be deposited later. For synonymy and a detailed discussion of the genus, see Shear (2020 (link)Shear ( , 2021a)) . Diagnosis: This species cannot be confused with any other, due to the unique modification of the tarsi of male legpairs 5 and 6, which are enormously swollen and pyriform (Figs 3, 6, 7) .
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