Total proteins were extracted from cell lines in lysis buffer containing 50 mM Tris (pH 6.8), 100 mM dithiothreitol, 1 % sodium dodecyl sulfate, and 25 % glycerol. Equal amount of proteins were electrophoresed by 12 % SDS–polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride (PVDF) membranes using the Mini-PROTEAN 3 system (Bio-Rad, Hercules, USA). PVDF membranes were blocked in 5 % bovine serum albumin and incubated with rabbit antihuman Prdx-6 polyclonal antibody (1:2000; Abcam, Cambridge, MA, USA) followed by horseradish peroxidase-linked goat–antimouse IgG (Proteintech, Chicago, IL, USA). β-Actin was used as a loading control (Sigma, St. Louis, MO, USA). Protein intensity was quantified using the LANE 1D Analyzer software (Sage Creation, Beijing, China).
Lane 1d analyzer software
Manufactured by Sagecreation
Sourced in China
The LANE 1D Analyzer software is a tool used for the analysis of one-dimensional gel electrophoresis data. It provides basic functionality for visualizing, quantifying, and comparing protein or nucleic acid bands in gel images.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using lane 1d analyzer software
1
Quantitative Immunoblotting of Prdx-6
2
Profiling Checkpoint Proteins in Cell Cycle
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Protein lysates were separated by 12% Tris-glycine polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride (PVDF) membranes (GE Healthcare; Little Chalfont, UK). Membranes were incubated with mouse monoclonal antibody against human Chk1, p-Chk1 (Abcam; Cambridge, UK), CDC25A, CyclinB1 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), followed by horseradish peroxidase (HRP)-labeled goat-anti-mouse IgG (PTGLab; Chicago, IL, USA). β-actin (Sigma Chemicals; St. Louis, MO, USA) was used as a protein loading control. The HRP conjugate was detected by a chemioluminescence ECL kit (Amersham) and autofluorography. Protein intensity was quantified using the LANE 1D Analyzer software (Sage Creation, Beijing, China).
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