The experimental fish were fasted for 24 h after the 4-week experimental feeding period, and 3 fish were randomly taken from each tank (n = 4 × 3 fish/treatment). The collected fish were dissected to separate out the whole intestine and scratch out the intestinal content with scissors and a blade, which was then washed in PBS buffer 3 times, and finally the liquid on the intestine was blotted with filter paper. Subsequently, the whole intestine was cut into pieces with sterile scissors and put into a 1.5 mL RNase free sample collection tube (Axygen, MCT-150-C-S, Union City, CA, USA). Finally, samples were immediately frozen in liquid nitrogen and stored at −80 °C for use.
Mct 150 c s
Manufactured by Corning
Sourced in United States
The MCT-150-C-S is a laboratory equipment product manufactured by Corning. It serves as a centrifuge device, designed to separate different components of a liquid sample through the application of centrifugal force.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mct 150 c s
1
Intestinal Sample Preparation for RNA Analysis
2
Peripartum Blood and Milk Sampling Protocol
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Blood samples were obtained from the coccygeal vein at around 0930 h -1000 h once a week at weeks -4, -3, -2, -1, 1, 2, 3, and 4 relative to calving. The blood was collected into 2 mL EDTA anticoagulant vacutainer tubes (EDTA k2), and then sent to an animal hospital (Nargis Animal Hospital, Hangzhou, China) within 2 h for analysis of blood variables. Another blood sample was collected into 5 mL vacutainer tubes (Yuli Medical Instrument Co., Jiangsu, China) and then kept at 4°C until separation of serum. Clotted blood was centrifuged at 3000 × g for 15 min. The serum was transferred to a sterile 1.5 mL RNase-free plastic test tube (MCT-150-C-S, Axygen, Corning, NY, USA), and then stored at -80°C until analysis.
Milk yield was recorded daily during the sampling periods, and milk samples were collected once a week at weeks 1, 2, 3, and 4 with milk-sampling devices (Waikato Milking Systems NZ Ltd., Hamilton, New Zealand). Daily samples were composited proportionally (4:3:3 according to three times of milking) and stored with added bronopol tablets (milk preservative; D & F Control System Inc., San Ramon, CA, USA) for later analysis of milk compositions.
Milk yield was recorded daily during the sampling periods, and milk samples were collected once a week at weeks 1, 2, 3, and 4 with milk-sampling devices (Waikato Milking Systems NZ Ltd., Hamilton, New Zealand). Daily samples were composited proportionally (4:3:3 according to three times of milking) and stored with added bronopol tablets (milk preservative; D & F Control System Inc., San Ramon, CA, USA) for later analysis of milk compositions.
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