After 96 and 144 hours of treatments, cells were lysed in AlphaLISA lysis buffer (Perkin Elmer, Waltham, MA USA) and stored at −80 °C until use. Cell lysates were diluted 1:10 in Alpha Immunoassay buffer and collagen content was quantified using COL1A1 AlphaLISA Detection kit (Perkin Elmer, Waltham, MA USA) according to the manufacturer’s instructions. Results were obtained by fitting data to a dose-response sigmoidal curve generated with Graph Pad Prism® version 5.01 and normalized to total protein.
Col1a1 alphalisa detection kit
Manufactured by PerkinElmer
Sourced in United States
The COL1A1 AlphaLISA Detection kit is a bead-based assay that measures the concentration of COL1A1, a protein involved in collagen formation. The kit uses AlphaLISA technology to detect and quantify COL1A1 levels in biological samples.
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Lab products found in correlation
2 protocols using col1a1 alphalisa detection kit
1
Quantifying Collagen in Cell Lysates
2
Collagen Quantification in Cell Lysates
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After 96 and 144 h of treatment, cells were lysed in AlphaLISA lysis buffer (Perkin Elmer) and stored at −80 °C until use. The cell lysate was diluted 1:10 v/v in Alpha Immunoassay buffer (Perkin Elmer) and collagen content was quantified using COL1A1 AlphaLISA Detection kit (Cat#AL371C, Perkin Elmer) according to the manufacturer’s instructions. Results were obtained fitting the data to a doseresponse sigmoidal curve generated with Graph Pad Prism® version 5.01 (GraphPad Software, Inc., La Jolla, CA, USA) and normalized to the total protein concentration.
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