Deae sepharose fast flow deae qff

Dendrobium nobile Lindl. was purchased from Guizhou Chishui Guo Li Dendrobium Development Co., Ltd. (Chishui, China). After drying and crushing, it was sieved through 80 meshes and left on standby. Ten kinds of standard monosaccharides, such as mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose, and fucose, were purchased from Aladdin Reagent Co., Ltd. (Shanghai, China). ELISA kits (including TNF-α, IL-1β, IL-6 and IL-10) were purchased from Shanghai Jianglai Biological Co., Ltd. (Shanghai, China). DEAE-Sepharose Fast Flow (DEAE-QFF) and Sephacryl S-300 HR were purchased from GE Healthcare. Other chemicals and reagents were of analytical grade.

Dendrobium officinale was purchased from Longanba Green Spring Agricultural Development Co., Ltd. (Guiding, China). The samples were dried at 60 °C, crushed, passed through an 80-mesh sieve and set aside.
Saccharomyces cerevisiae FBKL2.8022 (Sc) and Wickerhamomyces anomalous FBKL2.8023 (Wa) were derived from the Key Laboratory of Fermentation Engineering and Biological Pharmacy of Guizhou Province. The CCTCC NO. was M2019406 and M2019412, respectively [13 (link)]. Monosaccharide standards were purchased from Aladdin Reagent Co., Ltd. (Shanghai, China), including mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose, arabinose, and fucose. Amyloglucosidase and anhydrous D-glucose were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). NO kits were purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China). TNF-α, IL-1β, IL-6, and IL-10 ELISA kits were purchased from Shanghai Jianglai Biological Co., Ltd. (Shanghai, China). The relative Mw of polysaccharides was calculated according to calibration curves of the dextran standards purchased from Sigma-Aldrich (St. Louis, MO, USA). DEAE-Sepharose Fast Flow (DEAE-QFF) and Sephacryl S-300 HR were purchased from GE Healthcare. A G4000SWXL column (7.8 × 300 mm, Tosoh Co., Ltd., Tokyo, Japan) was used. The other chemicals and reagents were analytical grade.