Envision polymer hrp 3 3 diaminobenzidine dab

Manufactured by Agilent Technologies

Envision Polymer-HRP 3,3′diaminobenzidine (DAB) is a lab equipment product from Agilent Technologies. It is a horseradish peroxidase (HRP) conjugated polymer that can be used for immunohistochemistry (IHC) staining of tissue samples.

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Lab products found in correlation

Anti ki67 rabbit monoclonal antibody, by Cell Signaling Technology (1 mentions) Signalstain boost ihc detection reagent, by Cell Signaling Technology (1 mentions) Hrp conjugated anti rabbit secondary antibody, by Cell Signaling Technology (1 mentions) Cd3 polyclonal, by Agilent Technologies (1 mentions)

Close spelling variants of this product

DAB (905 citations) Diaminobenzidine (DAB) (112 citations) EnVision/HRP (63 citations) EnVision polymer (27 citations) DAB+ (3,3′-diaminobenzidine; (22 citations) Polymer-HRP (3 citations) Envision Polymer-HRP anti-mouse/3,3′diaminobenzidine (DAB, (3 citations)

2 protocols using envision polymer hrp 3 3 diaminobenzidine dab

Immunohistochemical Analysis of Ki67 Expression

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Tissue sections on charged glass slides were cut to 5 μm and deparaffinized in xylene and rehydrated via a stepped-gradient in ethanol. Peroxidase blocking, heat-induced antigen retrieval, and primary antibody incubation were performed per standard protocol under the following abbreviated conditions: anti-Ki67 rabbit monoclonal antibody (Cell Signaling; cat # 9129; 1:400), with citrate (pH 6.0) and SignalStain^® Boost IHC detection reagent. The primary antibody was incubated at room temperature for 1 hour followed by standard chromogenic staining with the Envision Polymer-HRP 3,3′diaminobenzidine (DAB; Dako) process. HRP-conjugated anti-rabbit secondary antibody (Cell Signaling; cat # 8114) was used to develop the signal. Qualitative immunohistochemistry scoring was performed by a pathologist who was unaware of the treatment that the animals received. All immunohistochemistry results were evaluated against positive and negative controls. Tissue sections from the same blocks were used for H&E staining using standard protocol.

Hebert K.A., Jaramillo S., Yu W., Wang M., Veeramachaneni R., Sandulache V.C., Sikora A.G., Bonnen M.D., Annapragada A.V., Corry D., Kheradmand F., Pandita R.K., Ludwig M.S., Pandita T.K., Huang S., Coarfa C., Grimm S.L., Perera D., Miles G, & Ghebre Y.T. (2021). Esomeprazole enhances the effect of ionizing radiation to improve tumor control. Oncotarget, 12(14), 1339-1353.

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Immunohistochemical Analysis of CD3+ TILs

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Cut tissue sections (5 μm) on charged glass slides were baked for 10-12 hours at 58°C in a dry slide incubator, deparaffinized in xylene and rehydrated via an ethanol step gradient. Heat-induced antigen retrieval steps were performed at pH 9.0, with the primary antibody incubated at room temperature for 1 hour (CD3, polyclonal, Dako, 1:100) followed by standard chromogenic staining protocol with the Envision Polymer-HRP/3,3’diaminobenzidine (DAB, Dako) process. Slides were counterstained in Harris hematoxylin. Immunohistochemistry scoring was performed using the percentage of stromal CD3-positive tumor infiltrating lymphocytes (TILS). All IHC results were evaluated against positive and negative tissue controls.

Huang C., Chen L., Savage S.R., Eguez R.V., Dou Y., Li Y., da Veiga Leprevost F., Jaehnig E.J., Lei J.T., Wen B., Schnaubelt M., Krug K., Song X., Cieślik M., Chang H.Y., Wyczalkowski M.A., Li K., Colaprico A., Li Q.K., Clark D.J., Hu Y., Cao L., Pan J., Wang Y., Cho K.C., Shi Z., Liao Y., Jiang W., Anurag M., Ji J., Yoo S., Zhou D.C., Liang W.W., Wendl M., Vats P., Carr S.A., Mani D.R., Zhang Z., Qian J., Chen X.S., Pico A.R., Wang P., Chinnaiyan A.M., Ketchum K.A., Kinsinger C.R., Robles A.I., An E., Hiltke T., Mesri M., Thiagarajan M., Weaver A.M., Sikora A.G., Lubiński J., Wierzbicka M., Wiznerowicz M., Satpathy S., Gillette M.A., Miles G., Ellis M.J., Omenn G.S., Rodriguez H., Boja E.S., Dhanasekaran S.M., Ding L., Nesvizhskii A.I., El-Naggar A.K., Chan D.W., Zhang H, & Zhang B. (2021). Proteogenomic insights into the biology and treatment of HPV-negative head and neck squamous cell carcinoma. Cancer cell, 39(3), 361-379.e16.

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