The frequency of activated CD4+ T cells was determined by staining spleen cells from BDC and BDC-Idd9.905 with anti-CD4 (RM4-5) mAb and anti-CD25 (7D4), -CD62 ligand (MEL-14), -CD69 (H1.2F3) or -CD44 (IM7.8.1) mAb (BD Bioscience), followed by flow cytometric analyses as described (7 (link)). Treg cell frequency was determined by surface staining of spleen cells with anti-CD4 (RM4-5) mAb and intracellular staining with anti-Foxp3 (MF23) mAb using reagents from BD Bioscience. Stained cells were acquired on a FACS Calibur or LSR II flow cytometer (Becton Dickinson) and analyzed using FlowJo software (Tree Star).
Anti cd4 rm4 5 mab
Manufactured by BD
Anti-CD4 (RM4-5) mAb is a monoclonal antibody that binds to the CD4 surface antigen. It is a laboratory tool used for the identification and analysis of CD4-positive cells.
Automatically generated - may contain errors
Lab products found in correlation
1 protocol using anti cd4 rm4 5 mab
1
Flow Cytometric Analysis of Activated T Cell Subsets
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