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Vero cells opti sfm

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Vero cells (OPTI-SFM) are a type of cell line derived from the kidney of the African green monkey. They are commonly used in cell culture and research applications. The OPTI-SFM medium is a serum-free formulation designed to support the growth and maintenance of Vero cells.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)

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Vero cells (55 citations) OPTI-SFM (3 citations)

2 protocols using vero cells opti sfm

1

Viral Titer Determination Protocol

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The M1 and GFP-labeled M1 used in this study were grown in Vero cells (OPTI- SFM, 12309-019, Thermo Fisher, Waltham, MA). Viral titers in BHK-21 cells were determined via TCID50 assay and converted to PFUs.
Hu C., Liu Y., Lin Y., Liang J.K., Zhong W.W., Li K., Huang W.T., Wang D.J., Yan G.M., Zhu W.B., Qiu J.G, & Gao X. (2018). Intravenous injections of the oncolytic virus M1 as a novel therapy for muscle-invasive bladder cancer. Cell Death & Disease, 9(3), 274.
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2

Culturing Cells for Virus Development

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The cells were cultured at 37°C, 5% CO2, and complemented with 10% (v/v) fetal bovine serum plus 1% penicillin/streptomycin (Life Technologies, China). Cell lines were purchased from the American Type Culture Collection (Maryland, USA) and the Shanghai Institute of Cell Biology. All cell lines used in this study were listed in Supplementary Table. M1 virus was developed in Vero cells (OPTI-SFM, 12309-019, Thermo Fisher, USA) and collected for experiments. M1 virus titers were detected by TCID50 assay by utilizing BHK-21 cells. The data were converted to plaque-forming units (PFU) according to the converting equation from ATCC (https://www.atcc.org/en/Global/FAQs/4/8/Converting_TCID50_to_plaque_forming_units_PFU-124.aspx) that was TCID50 titer (per mL) by 0.7 to predict the mean number of PFU.
Liu Y., Li K., Zhu W.B., Zhang H., Huang W.T., Liu X.C., Lin Y., Cai J., Yan G.M., Qiu J.G., Peng L., Liang J.K, & Hu C. (2020). Suppression of CCDC6 sensitizes tumor to oncolytic virus M1. Neoplasia (New York, N.Y.), 23(1), 158-168.
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