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H l igg

Manufactured by Thermo Fisher Scientific
Sourced in United States

The H + L IgG is a laboratory equipment product designed to detect the presence of immunoglobulin G (IgG) antibodies in biological samples. It functions as a detection reagent, providing a reliable method to identify IgG levels.

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3 protocols using h l igg

1

Immunostaining Detection Protocols

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Detection of immunostaining was carried out using horseradish peroxidase–diaminobenzidine (see above) for immunohistochemistry and secondary antibodies conjugated with Alexa Fluor® 488 (1:1000, donkey anti-rabbit, H + L IgG, Thermo Fischer Scientific, Waltham, ME, USA) and Alexa Fluor® 568 (1:1000, donkey anti-mouse, H + L IgG, Thermo Fischer Scientific, Waltham, ME, USA) for immunofluorescence staining. Slides incubated with only the secondary antibody were used as specificity controls.
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2

Immunohistochemical and Immunofluorescence Staining

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Detection of immunostaining was carried out using horseradish peroxidase–diaminobenzidine (see above) for immunohistochemistry and secondary antibodies conjugated with Alexa Fluor® 488 (1:1000, donkey anti-rabbit, H + L IgG, Thermo Fischer Scientific, Waltham, MA, USA) and Alexa Fluor® 568 (1:1000, donkey anti-mouse, H + L IgG, Thermo Fischer Scientific, Waltham, MA, USA) for immunofluorescence staining. Slides incubated with only the secondary antibody were used as specificity controls.
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3

Immunostaining Detection Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
Detection of immunostaining was carried out using horseradish peroxidase–diaminobenzidine (see above) for immunohistochemistry and secondary antibodies conjugated with Alexa Fluor® 488 (1:1000, donkey anti-rabbit, H + L IgG, Thermo Fischer Scientific, Waltham, MA, USA) and Alexa Fluor® 568 (1:1000, donkey anti-mouse, H + L IgG, Thermo Fischer Scientific) for immunofluorescence staining. Slides incubated with only the secondary antibody were used as specificity controls.
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