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Amicon ultra 4 spin filter tube

Manufactured by Merck Group
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The Amicon Ultra-4 spin filter tube is a laboratory centrifugal device used for the concentration and purification of macromolecules, such as proteins, from solutions. The device utilizes a semi-permeable membrane to selectively retain the desired macromolecules while allowing smaller molecules and solvents to pass through during the centrifugation process.

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Lab products found in correlation

Ec plan neofluar 100x 1.3 objective, by Zeiss (2 mentions) Axio observer z1, by Zeiss (2 mentions) Ktapurifier 10 fplc system, by GE Healthcare (2 mentions) Histrap hp column, by Cytiva (2 mentions) 0.22 m pes filter, by Techno Plastic Products (2 mentions)

Spelling variants of this product

Amicon Ultra (740 citations) Amicon Ultra-4 (263 citations) Amicon filters (181 citations) Amicon Ultra filters (132 citations) Amicon tubes (38 citations) Amicon spin filters (21 citations) Amicon Ultra tubes (15 citations) Spin filter (15 citations) Amicon filter tube (3 citations) Amicon Ultra-4 tube (2 citations)

4 protocols using amicon ultra 4 spin filter tube

1

Time-lapse Imaging of Bacterial Cell Dynamics

Check if the same lab product or an alternative is used in the 5 most similar protocols
Leaf245 was grown in R-2A+M broth and the supernatant was concentrated
200-fold with an Amicon Ultra-4 spin filter tube (100 kDa, Merck). Leaf374 grown
on R-2A+M agar was resuspended in PBS pH7.4 (10 mM
Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl,
2.7 mM KCl), spotted on a LB agar pad and dried. The concentrated supernatant of
Leaf 245 was added (10 μL) and imaging was started one minute later.
Phase contrast images were acquired on a Zeiss Axio Observer Z1 inverted
microscope (Carl Zeiss AG) equipped with an EC Plan-Neofluar 100x/1.3 objective.
Images were taken every 20 seconds over the course of 20 minutes and combined to
a time lapse video using Fiji software78 (link).
Schäfer M., Vogel C.M., Bortfeld-Miller M., Mittelviefhaus M, & Vorholt J.A. (2022). Mapping phyllosphere microbiota interactions in planta to establish genotype-phenotype relationships. Nature microbiology, 7(6), 856-867.
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2

His-Tagged Protein Purification by FPLC

Check if the same lab product or an alternative is used in the 5 most similar protocols
Affinity purification was carried out on an ÄKTA Purifier 10 FPLC
system (GE healthcare). Cleared cell lysate (4-6 mL) was passed through a 0.22
µm PES filter (TechnoPlasticProducts) and loaded on a HisTrap™ HP
column (1 mL, Cytiva) equilibrated with 20 mM TRIS-HCl pH 7.5 containing 250 mM
NaCl and 40 mM imidazole. The column was washed with 10 column volumes of the
same buffer and bound proteins were eluted over a gradient of 10 mL to a final
concentration of 500 mM imidazole. Elution of proteins was monitored by
absorption at 215 and 280 nm and 1 mL fractions were collected. Based on
SDS-PAGE gel band pattern, fractions containing purified protein were pooled and
imidazole concentration was reduced by 20-fold dilution in buffer without
imidazole followed by volume reduction with an Amicon Ultra-4 spin filter tube
(3 kDa, Merck).
Schäfer M., Vogel C.M., Bortfeld-Miller M., Mittelviefhaus M, & Vorholt J.A. (2022). Mapping phyllosphere microbiota interactions in planta to establish genotype-phenotype relationships. Nature microbiology, 7(6), 856-867.
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3

His-Tagged Protein Purification by FPLC

Check if the same lab product or an alternative is used in the 5 most similar protocols
Affinity purification was carried out on an ÄKTA Purifier 10 FPLC
system (GE healthcare). Cleared cell lysate (4-6 mL) was passed through a 0.22
µm PES filter (TechnoPlasticProducts) and loaded on a HisTrap™ HP
column (1 mL, Cytiva) equilibrated with 20 mM TRIS-HCl pH 7.5 containing 250 mM
NaCl and 40 mM imidazole. The column was washed with 10 column volumes of the
same buffer and bound proteins were eluted over a gradient of 10 mL to a final
concentration of 500 mM imidazole. Elution of proteins was monitored by
absorption at 215 and 280 nm and 1 mL fractions were collected. Based on
SDS-PAGE gel band pattern, fractions containing purified protein were pooled and
imidazole concentration was reduced by 20-fold dilution in buffer without
imidazole followed by volume reduction with an Amicon Ultra-4 spin filter tube
(3 kDa, Merck).
Schäfer M., Vogel C.M., Bortfeld-Miller M., Mittelviefhaus M, & Vorholt J.A. (2022). Mapping phyllosphere microbiota interactions in planta to establish genotype-phenotype relationships. Nature microbiology, 7(6), 856-867.
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4

Time-lapse Imaging of Bacterial Cell Dynamics

Check if the same lab product or an alternative is used in the 5 most similar protocols
Leaf245 was grown in R-2A+M broth and the supernatant was concentrated
200-fold with an Amicon Ultra-4 spin filter tube (100 kDa, Merck). Leaf374 grown
on R-2A+M agar was resuspended in PBS pH7.4 (10 mM
Na2HPO4, 1.8 mM KH2PO4, 137 mM NaCl,
2.7 mM KCl), spotted on a LB agar pad and dried. The concentrated supernatant of
Leaf 245 was added (10 μL) and imaging was started one minute later.
Phase contrast images were acquired on a Zeiss Axio Observer Z1 inverted
microscope (Carl Zeiss AG) equipped with an EC Plan-Neofluar 100x/1.3 objective.
Images were taken every 20 seconds over the course of 20 minutes and combined to
a time lapse video using Fiji software78 (link).
Schäfer M., Vogel C.M., Bortfeld-Miller M., Mittelviefhaus M, & Vorholt J.A. (2022). Mapping phyllosphere microbiota interactions in planta to establish genotype-phenotype relationships. Nature microbiology, 7(6), 856-867.
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+ Expand

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