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Dna primers

Manufactured by Eurofins
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Sourced in Germany, United States

DNA primers are short, synthetic DNA sequences used as starting points for DNA synthesis and amplification in various molecular biology techniques. They serve as essential components in processes such as polymerase chain reaction (PCR), DNA sequencing, and genetic analysis.

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Lab products found in correlation

Qiaquick gel extraction kit, by Qiagen (3 mentions) Qiaprep spin miniprep kit, by Qiagen (3 mentions) Dna modifying enzymes, by New England Biolabs (3 mentions) Protease and phosphatase inhibitor mini tablets, by Roche (2 mentions) Quikchange lightning site directed mutagenesis kit, by Agilent Technologies (1 mentions) Biotin maleimide, by Merck Group (1 mentions) Biotin 4 fluorescein, by Biotium (1 mentions) Neb builder kit, by New England Biolabs (1 mentions) Opti 4cn detection kit, by Bio-Rad (1 mentions) D galactose, by Merck Group (1 mentions) Growth media and chemicals, by Avantor (1 mentions) Plasmid mini kit 1, by Omega Bio-Tek (1 mentions) Cy7 maleimide, by Lumiprobe (1 mentions) Instantblue protein stain, by Abcam (1 mentions) Nebuilder hifi dna assembly master mix, by New England Biolabs (1 mentions) Nanodrop spectrophotometer, by Thermo Fisher Scientific (1 mentions) Water purification system, by Merck Group (1 mentions) E coli bl21 λ de3, by Agilent Technologies (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Aniline, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Custom DNA mutagenesis primers (3 citations)

9 protocols using dna primers

1

Versatile DNA Manipulation Techniques

Cited 1 time
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Techniques for
plasmid DNA preparation, restriction digestion, transformation, and
DNA gel electrophoresis were adapted from standard protocols.21 Isolation of genomic DNA was carried out based
on the manufacturer’s instructions (QIAGEN). PCR amplifications
were carried out on thermocyclers (SensoQuest). Restriction enzymes
and DNA polymerases (Taq, Q5, and Phusion) were purchased from New
England Biolabs or Thermo Fisher Scientific. DNA primers were purchased
from Eurofins MWG Operon. The general plasmids used in this work are
listed in Table S2. The PCR primers used
in this study are shown in Table S4. All
plasmids generated in this study (Table S4) were constructed using Hot Fusion Cloning.22 (link)
Abebew D., Sayedain F.S., Bode E, & Bode H.B. (2022). Uncovering Nematicidal Natural Products from Xenorhabdus Bacteria. Journal of Agricultural and Food Chemistry, 70(2), 498-506.
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2

Recombinant Protein Expression Protocol

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Reagents for buffer preparation were purchased from Fisher Scientific, unless otherwise specified. DNA primers were purchased from Eurofins MWG Operon. Chemically competent E.coli cells BL21(λDE3) were purchased from Lucigen or Novagen. QuikChange Lightning Site-Directed Mutagenesis kit was purchased from Agilent Technologies. Biotin-4-fluorescein was purchased from Biotium. Biotin-maleimide and streptavidin was purchased from Sigma Aldrich. Protease and phosphatase inhibitor mini tablets were purchased from Roche. For gene assembly, NEB builder kit was purchased from New England Biolabs. For Immunoblot, Opti-4CN detection kit was purchased from Biorad.
Sharma J., Uchida M., Miettinen H.M, & Douglas T. (2017). Modular interior loading and exterior decoration of a virus-like particle. Nanoscale, 9(29), 10420-10430.
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3

Cloning and Protein Expression Protocol

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All enzymes for cloning were purchased from NEB (Beverly, MA). DNA primers were ordered from Eurofins Genomics LLC (Louisville, KY) or GENEWIZ Inc. (Cambridge, MA). Growth media and chemicals were purchased from Amresco (Solon, OH) or RPI Corp (Mount Prospect, IL). High-purity l-arabinose and d-galactose were purchased from Sigma-Aldrich (St. Louis, MO). Plasmid Mini Kit I, PCR Purification and Gel Extraction Kits were obtained from Omega Bio-tek (Norcross, GA). Mouse-anti-His6 primary antibody (MA1–21315) and goat anti-mouse IgG H&L (Alexa Fluor® 488) (A1101) were purchased from Thermo Fisher Scientific (Waltham, MA).
Bober J.R, & Nair N.U. (2019). Galactose to tagatose isomerization at moderate temperatures with high conversion and productivity. Nature Communications, 10, 4548.
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4

Molecular Cloning and Protein Expression

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DNA modifying enzymes were purchased from New England Biolabs and Promega. DNA primers were purchased from Eurofins MWG Operon. Escherichia coli chemically competent BL21(λDE3) and electro competent ClearColi cells were purchased from Lucigen. QIAquick gel extraction kit and QIAprep Spin Miniprep kit were purchased from Qiagen. Cy7-maleimide was purchased from LumiProbe. All other chemical reagents were purchased from Fisher Scientific.
Schwarz B., Madden P., Avera J., Gordon B., Larson K., Miettinen H.M., Uchida M., LaFrance B., Basu G., Rynda-Apple A, & Douglas T. (2015). Symmetry Controlled, Genetic Presentation of Bioactive Proteins on the P22 Virus-like Particle Using an External Decoration Protein. ACS nano, 9(9), 9134-9147.
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5

Bacterial Strains and Cultivation Conditions

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A list of bacterial strains and plasmids used in this study is provided in Table 1. P. aeruginosa PAO1 was either grown in complex Luria Bertani (LB) Miller broth (Carl Roth, Karlsruhe, Germany) or in BM2 minimal medium [62 mM potassium phosphate buffer, pH 7.0, 7 mM (NH4)2SO2, 2 mM MgSO4, 10 μM FeSO4, 0.4% (w/v) Glucose] at 37°C and 170 rpm. In BM2-swarm medium, 7 mM (NH4)2SO4 was substituted for 0.5% (w/v) casamino acids. Escherichia coli strains DH5α and BL21(DE3) were cultivated in LB medium at 37°C. For overexpression experiments, the arabinose-inducible expression vector pJN105 and the IPTG-inducible vector pET23a were used. Antibiotics ampicillin (100 μg/ml) and gentamicin (10 μg/ml for E. coli; 30 μg/ml for P. aeruginosa) were added for plasmid selection and/or maintenance.
Hypochlorite was added in the form of aqueous sodium hypochlorite (NaClO) or calcium hypochlorite [Ca(ClO)2] solutions as mentioned within the text. For effects caused by both, NaClO and Ca(ClO)2 treatment, the general term HClO was used within the manuscript.
Free chlorine concentrations of HClO solutions (VWR, Darmstadt, Germany) were determined prior to each assay using DPD Free Chlorine Powder Pillows (VWR, Darmstadt, Germany) according to the manufacturer’s instructions.
The sequences of DNA primers (Eurofins MWG Operon, Germany) used in these studies are shown in Supplementary Tables S1 and S2.
Strempel N., Nusser M., Neidig A., Brenner-Weiss G, & Overhage J. (2017). The Oxidative Stress Agent Hypochlorite Stimulates c-di-GMP Synthesis and Biofilm Formation in Pseudomonas aeruginosa. Frontiers in Microbiology, 8, 2311.
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6

Recombinant Protein Expression Techniques

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DNA modifying enzymes were purchased from New England Biolabs (Ipswich, MA). DNA primers were purchased from Eurofins MWG Operon (Huntsville, AL). E. coli BL21(λDE3) and 10G E. cloni electrocompotent cells were purchased from Lucigen (Madison, WI). QIAquick gel extraction kit, MinElute Enzyme Reaction Cleanup kit, and QIAprep Spin Miniprep kit were purchased from Qiagen (Valencia, CA). InstantBlue protein stain was purchased from Expedeon (San Deigo, CA). All chemical reagents were purchased from Fisher Scientific (Hampton, NH).
Patterson D., Schwarz B., Avera J., Western B., Hicks M., Krugler P., Terra M., Uchida M., McCoy K, & Douglas T. (2017). Sortase-Mediated Ligation as a Modular Approach for the Covalent Attachment of Proteins to the Exterior of the Bacteriophage P22 Virus-like Particle. Bioconjugate chemistry, 28(8), 2114-2124.
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7

Streamlined Recombinant Protein Expression

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Reagents for buffer preparation were purchased from Fisher Scientific, unless otherwise specified. DNA primers were purchased from Eurofins MWG Operon. E. coli BL21(λDE3) were purchased from Lucigen. Protease and phosphatase inhibitor mini tablets were purchased from Roche. For gene assembly, NEBuilder® HiFi DNA assembly master mix was purchased from New England Biolabs. Gene block were purchased from IDT.
Sharma J, & Douglas T. (2019). Tuning the catalytic properties of P22 nanoreactors through compositional control. Nanoscale, 12(1), 336-346.
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8

Protein Expression and Purification

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DNA modifying enzymes were purchased from New England Biolabs and Thermo Fisher Scientific. DNA primers were purchased from Eurofins and Sigma. E. coli BL21 (λ DE3) was purchased from Agilent Technologies. QIAquick gel extraction kit and QIAprep spin miniprep kits were purchased from Qiagen. Ancillary reagents and EDTA [2,2′,2″,2′′′-(Ethane-1,2-diyldinitrilo) tetra acetic acid] were purchased from Sigma. All other chemical reagents used were either purchased from Sigma or Fisher Scientific, used as received unless otherwise specified. Water was purified using a Merck Millipore water purification system. Protein concentrations were determined by measuring absorbance at 280 nm using a NanoDrop spectrophotometer (Thermo Scientific).
Kumar M ., Markiewicz-Mizera J ., Janna Olmos JD ., Wilk P ., Grudnik P ., Biela AP ., Jemioła-Rzemińska M ., Górecki A ., Chakraborti S , & Heddle JG . (2021). A single residue can modulate nanocage assembly in salt dependent ferritin. Nanoscale, 13(27).
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9

Aniline-Ammonium Peroxydisulfate Synthesis

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Aniline and ammonium peroxydisulfate were purchased from Fisher Scientific, USA. Sodium dodecylbenzenesulfonate (Na-DBS) was purchased from Pfaltz and Bauer, U.S.A. Chloroform was obtained from EMD Chemicals, U.S.A. All the reagents were analytical grade and used without purification. Water used in all measurements and solutions was DNase/RNase-free distilled water. DNA primers were obtained from Eurofins MWG Operon, U.S.A. DNA sequences are the following:
Phosphate-buffered saline (PBS) was made using preformulated tablets (Fisher Scientific). Urea-polyacrylamide gels were made using the UreaGel system from national diagnostics. Total RNA extraction reagents used the TRI-reagent method (MRC).
Sengupta P.P., Gloria J.N., Amato D.N., Amato D.V., Patton D.L., Murali B, & Flynt A.S. (2015). Utilizing Intrinsic Properties of Polyaniline to Detect Nucleic Acid Hybridization through UV-Enhanced Electrostatic Interaction. Biomacromolecules, 16(10), 3217-3225.
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