Anti nut antibody clone c52b1

The three NC cases were retrieved from the consultation files of three of the authors (A.A., I.F. and C.F.). They have been diagnosed in the years 2010, 2015 and 2017. Immunohistochemistry (IHC) was performed on 3-μm sections cut from paraffin blocks using a fully automated system (“Benchmark XT System”, Ventana Medical Systems Inc, 1910 Innovation Park Drive, Tucson, Arizona, USA) and the following antibodies: pankeratin (clone AE1/AE3, 1:40, Zytomed, Berlin, Germany), CK7 (OV-TL, 1:1000, Biogenex), p63 (SSI6, 1: 100, DCS), S100 protein (polyclonal, 1:2500, Dako), CD56 (clone MRQ-42, 1:100, CELL MARQUE), chromogranin A (clone LK2H10, 1:500, Beckman-Coulter GmbH), synaptophysin (clone SY38, 1:50, Dako), SMARCB1 (MRQ-27, 1:50, Zytomed), and anti-NUT antibody (clone C52B1, 1:45, Cell Signaling). Interpretation of results of the NUT IHC was based on published data showing distinctive granular (punctate or dusty) nuclear immunoreactivity present in > 50% of neoplastic cell nuclei.^{18 (link)} Epstein Barr virus (EBV) in-situ hybridization (EBER 1/2 probes, ZytoVision, Bremerhaven, Germany) was performed according to the manufacturer guidelines. Positive and negative controls were used throughout. Normal testicular tissue was used as a positive control for the NUT IHC.^{5 (link),6 (link)}