R plex human neurofilament l antibody set

Manufactured by Mesoscale

The R-PLEX human neurofilament L antibody set is a laboratory equipment product designed for the detection and quantification of neurofilament L, a structural protein found in neurons. The set includes a capture antibody and a detection antibody, which can be used in a sandwich immunoassay format to measure the levels of neurofilament L in biological samples.

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Lab products found in correlation

Sandwich elisa kit, by Cloud-Clone (1 mentions)

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R-plex

3 protocols using r plex human neurofilament l antibody set

Plasma Aβ and NfL Biomarker Quantification

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Plasma Aβ₄₂ and Aβ₄₀ levels were assessed by immunoprecipitation mass spectrometry as previously described [11 ]. Aβ levels were analysed and calculated by integrated peak area ratios to known concentrations of the internal standards using the Skyline software package [12 ]. Plasma NfL levels were measured using the R-PLEX human neurofilament L antibody set (Meso Scale Discovery, F217X). Samples were diluted 2-fold in a diluent buffer and tested in duplicate according to the manufacturer’s instructions.
Plasma Aβ or NfL levels were measured once using blood samples collected in the first or second year of the study. For most participants (n = 420 out of 452), Aβ and NfL levels were measured from blood samples taken at the 1-year wave of data collection. For the rest of the participants, plasma markers were measured using samples from the second year. Blood samples were collected on the same day as cognitive and physical function evaluation.

He L., de Souto Barreto P., Aggarwal G., Nguyen A.D., Morley J.E., Li Y., Bateman R.J, & Vellas B. (2020). Plasma Aβ and neurofilament light chain are associated with cognitive and physical function decline in non-dementia older adults. Alzheimer's Research & Therapy, 12, 128.

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Serum Neurofilament Levels and Mortality

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Serum NfL levels were measured using the R-PLEX human neurofilament L antibody set (Meso Scale Discovery, F217X) in 96-well plate format. Samples were diluted 2-fold in diluent buffer and tested in duplicate according to the manufacturer's instructions. Samples from decedents and survivors were randomized across four batches in a blinded manner. The mean coefficient of variation of duplicate NfL measurements was 3.8% (0–27.5%). Vital status was determined through a National Death Index from 1/1/2000 through 12/31/2014. Deaths are coded as 1. Disease measures were based on self-report of physician-based diagnoses and included: hypertension, diabetes, cancer, COPD, heart attack, congestive heart failure, angina, asthma, arthritis, stroke, and kidney disease.

Nguyen A.D., Malmstrom T.K., Aggarwal G., Miller D.K., Vellas B, & Morley J.E. (2022). Serum neurofilament light levels are predictive of all-cause mortality in late middle-aged individuals. eBioMedicine, 82, 104146.

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CSF Biomarker Measurement Protocol

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We obtained 5-10 mL of CSF samples from the lumbar punctures of each study participant. Each sample was centrifuged at 4000×g for 10 min. Then, the supernatant was collected and immediately frozen in polypropylene tubes that were stored at -80 • C until assay. The biomarker assays were performed in duplicate by experienced technicians under blinded conditions, and the intra-and interassay coefficients of variation were <20%. The concentrations of Aβ42, t-tau, p-tau were measured using the Luminex xMAP platform (Luminex Corp, Austin, TX) with INNO-BIA AlzBio3 (Fujirebio Europe, Inc., Ghent, Belgium) as previously described [15] . The concentrations of NfL were measured using the R-PLEX Human Neurofilament L Antibody Set (Meso Scale Discovery, Rockville, MD). The concentrations of soluble NG2 and α-syn were measured using commercially available sandwich ELISA kits (Cloud Clone Corp. Katy, TX and Covance, Dedham, MA, respectively). We only used samples with hemoglobin (Hb) concentrations <1000 ng/mL for α-syn analysis because, as previously reported, blood contamination (Hb > 1000 ng/mL) might affect the level of CSF α-syn [4] .

Tokutake T., Kasuga K., Tsukie T., Ishiguro T., Shimohata T., Onodera O, & Ikeuchi T. (2022). Clinical correlations of cerebrospinal fluid biomarkers including neuron-glia 2 and neurofilament light chain in patients with multiple system atrophy. Parkinsonism & related disorders, 102.

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