OCT-embedded tissues were sectioned at 12 μm or 20 μm. Sections were rehydrated and blocked in antibody wash (5% heat inactivated goat serum, 0.1% Triton X-100 in Tris-Buffered Saline) for 45 minutes. Tissues were incubated with primary antibodies overnight at 4°C, washed three times with 0.1% Triton X-100 in Tris-Buffered Saline (TBST), and incubated with secondary antibodies for 1 hour at room temperature. Tissues were washed three times with TBST and incubated with Hoechst 33342 for 5 minutes. Slides were coverslipped with ProLong Gold (ThermoFisher) mounting media and imaged on a BioTek Lionheart FX microscope. Primary antibodies used were: mouse anti-ARL13B (1:1000, NeuroMab, N295B/66); rabbit anti-acetylated α-tubulin (1:1000,Cell Signaling, 5335); mouse anti-acetylated α-tubulin (1:2500, Sigma, T7451); chicken anti-ACIII (1:1000, Encor, CPCA-ACIII); rat anti-insulin (1:1000, R&D systems, MAB1417); rabbit anti-glucagon (1:2000, Abcam, ab92517). Secondary antibodies used were goat anti-mouse AlexaFluor 488, goat anti-chicken AlexaFluor 647, goat anti-rat AlexaFluor 568 and donkey anti-rabbit AlexaFluor 555 (1:500, ThermoFisher).
Rabbit anti acetylated α tubulin
Manufactured by Cell Signaling Technology
Rabbit anti-acetylated α-tubulin is a primary antibody that specifically recognizes the acetylated form of the α-tubulin protein. Acetylation of α-tubulin is a post-translational modification that can affect microtubule dynamics and function.
Automatically generated - may contain errors
Lab products found in correlation
Prolong gold, by Thermo Fisher Scientific (2 mentions)
Ab92517, by Abcam (1 mentions)
Lionheart fx microscope, by Agilent Technologies (1 mentions)
Mouse anti acetylated α tubulin, by Merck Group (1 mentions)
Alexa fluor 488 goat anti mouse, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 555 donkey anti rabbit, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 647 goat anti chicken, by Thermo Fisher Scientific (1 mentions)
Goat anti rat alexafluor 568, by Thermo Fisher Scientific (1 mentions)
Rabbit anti α tubulin monoclonal antibody, by Cell Signaling Technology (1 mentions)
Anti cdc42 antibody, by Abcam (1 mentions)
Anti phospho h2ax antibody, by Cell Signaling Technology (1 mentions)
Alexa fluor 594 donkey anti mouse, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 goat anti chicken, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 594 donkey anti rabbit, by Thermo Fisher Scientific (1 mentions)
Ab99379, by Abcam (1 mentions)
Tsc 5sp x confocal microscope, by Leica (1 mentions)
Alexa fluor 488 donkey anti mouse, by Thermo Fisher Scientific (1 mentions)
3 protocols using rabbit anti acetylated α tubulin
1
Immunolabeling of Ciliary and Endocrine Structures
2
Molecular Profiling of Oocyte Maturation
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About 300 oocytes of each group were briefly washed in phosphate-buffered saline and then lysed in 2× SDS buffer and stored at −80°C until use. Immunoblot analysis was preformed as described (Huang et al., 2015 (link)). For immunolabeling, the following primary antibodies and dilutions were used: goat anti–SPAG-1 antibody (1:200), anti–phospho-AMPKα antibody (1:1500), anti–phospho-cyclin B1 antibody (1:1000), anti–histone H3 (acetyl K9) antibody (1:1000), rabbit anti–cyclin B2 polyclonal antibody (1:200; Santa Cruz Biotechnology), rabbit anti–α-tubulin monoclonal antibody (1:1000; Cell Signaling Technology), rabbit anti–acetylated-α-tubulin (1:1000; Cell Signaling Technology), anti–phospho-H2A.X antibody (1:1000; Cell Signaling Technology), rabbit anti–phospho-p44/42 MAPK antibody (1:1000), rabbit anti-PKC monoclonal antibody (1:1000; Abcam), anti-CDC42 antibody (1:1000; Abcam), anti–N-WSAP antibody (1:1000; Abcam), anti-Arp3 antibody (1:1000; Abcam), and anti-BubR1 antibody (1:1000). The data were normalized to β-actin.
3
Multiprotein Centrosomal Complex Analyses
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Primary antibodies used were as follows: rabbit polyclonal anti-CEP164 was a gift from Erich Nigg (University of Basel, Switzerland), rabbit polyclonal anti-ERC1 (A302-698A, Bethyl Laboratories), rabbit polyclonal anti-CEP131 (ab99379, Abcam), rabbit polyclonal anti-RABEP2 (ab138279, Abcam), mouse monoclonal anti-SDCCAG8 (ab67098, Abcam), rabbit anti-DYKDDDDK Tag (#2368, Cell Signaling), mouse monoclonal anti-γ-tubulin (GTU-88, Sigma), rabbit anti-acetylated α-tubulin (#5335, Cell Signaling), mouse monoclonal anti-polyglutamylated tubulin (T9822, Sigma-Aldrich), rabbit polyclonal anti-CCDC41 (CEP83) (HPA038161, Atlas), rabbit polyclonal anti-FBF1 (HPA023677, Atlas), chicken anti-GFP (GFP-1020, Aves). Antibodies against FoxA2 (clone 4C7), Hb9 (clone 81.5C10), Nkx2.2 (clone 74.5A5) and Nkx6.1 (clone F65A2) are from the Developmental Studies Hybridoma Bank. Secondary antibodies were: donkey anti-mouse Alexa Fluor 488, donkey anti-mouse Alexa Fluor 594, donkey anti-rabbit Alexa Fluor 488 and donkey anti-rabbit Alexa Fluor 594 (Molecular Probes), goat anti-chicken Alexa Fluor 488 (Molecular Probes). Samples were mounted in ProlongGold (Molecular Probes) and images captured on a Leica TSC 5SP X confocal microscope (Leica Microsystems).
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