Opteia human il 6 elisa kit 2

Demographics, medical history, clinical data and main biological findings on the day of admission were collected from the electronic medical file. On the day of IL-6 blood concentration measurement, we also collected results of D-Dimers, C-Reactive Protein, white blood cells and lymphocytes count, lactate dehydrogenase (LDH) and ferritin as well as microbiological samples, whenever present. Data on ventilatory parameters (i.e. tidal volume, minute ventilation, positive end-expiratory pressure–PEEP, FiO₂ and PaO₂/FiO₂) and on the hemodynamic status (i.e. lactate, total and cardiovascular Sequential Organ Failure Assessment score) [9 ] were also collected. We also recorded the daily use of specific therapies, such as vasopressors, corticosteroids, prone positioning, the use of renal replacement therapy (RRT) or extra-corporeal membrane oxygenation (ECMO). Mortality was recorded at ICU discharge. Laboratory testing for IL-6 became available at the end of March 2020; routine IL-6 measurements were then performed for ICU patients on Monday and Thursday of each week. The IL-6 blood concentration was measured using an ELISA method from BD Biosciences (BD OptEIA Human IL-6 ELISA Kit II, San Jose, CA). All tests were performed according to the manufactory’s instructions. Serum samples were collected in serum separator tubes, centrifuged and stored at -20°C until assessment.