Brafca

Manufactured by Jackson ImmunoResearch

BRAFCA is a reagent used in immunohistochemistry and Western blotting applications. It is a recombinant protein that serves as a positive control for the detection of BRAF protein.

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Lab products found in correlation

Cd19 cre, by Jackson ImmunoResearch (1 mentions) Mx1 cre, by Jackson ImmunoResearch (1 mentions) Trp53flox flox, by Jackson ImmunoResearch (1 mentions) Ptenflox flox, by Jackson ImmunoResearch (1 mentions) Trp53flox, by Jackson ImmunoResearch (1 mentions) Etv1cre er t2, by Jackson ImmunoResearch (1 mentions)

2 protocols using brafca

Modeling BRAF-driven Cancers in Mice

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All mice strains were obtained from the Jackson laboratory, which include BRAF^CA/+ (#017837, a Cre-driven BRAF(V600E)-knockin strain) [58 (link)], Trp53^flox/flox (#008462), pTEN^flox/flox (#006440), p27^flox/flox (#027328), Mx1-cre (#003556), and cd19^cre/+ (#006785) mice strains. These strains were bred to generate BRAF^CA/+Mx1-cre, BRAF^CA/+ cd19^cre/+, BRAF^CA/+Trp53^flox/flox cd19^cre/+, BRAF^CA/+pTEN^flox/flox cd19^cre/+, BRAF^CA/+p27^flox/flox cd19^cre/+ mice strains. All mice strains were maintained in the animal facility at the National Cancer Center Singapore (NCCS). To track the disease progression in mice with different genetic modifications, 20 mice per strain were used and monitored up to 40 weeks. To characterize the pathological features of disease in mice, animals at the terminal stage of diseases were euthanized and sacrificed for harvesting of blood, spleen, liver, lung, skin and bone marrow according to standard protocols. All operations were approved by the institutional Animal Care and Use Ethics Committee of NCCS.

Yap J., Yuan J., Ng W.H., Chen G.B., Sim Y.R., Goh K.C., Teo J., Lim T.Y., Goay S.M., Teo J.H., Lao Z., Lam P., Sabapathy K, & Hu J. (2023). BRAF(V600E) mutation together with loss of Trp53 or pTEN drives the origination of hairy cell leukemia from B-lymphocytes. Molecular Cancer, 22, 125.

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Genetically Engineered Mouse Models for GI Tumors

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All mouse studies are conducted in accordance with MSKCC IACUC under protocol 11-12-029. The Braf^CA, Etv1^CreERT2, Trp53^flox and Rosa26^{CAGGS-LSL-EYFP} mice were obtained from The Jackson Laboratory. To trace the Etv1-expressing cellular compartments in the GI tract, Etv1^CreERT2/+; Rosa26^{CAGGS-LSL-EYFP/+} mice were generated through standard mouse breeding with Etv1^{CreERT2/CreERT2} and Rosa26^{CAGGS-LSL-EYFP/CAGGS-LSL-EYFP} mice. Etv1^CreERT2; Braf^CA/CA and Etv1^CreERT2; Braf^CA/+ mice were generated through breeding of Etv1^CreERT2 and Braf^CA/CA mice. To cross into the Trp53 conditional background, we crossed Etv1^CreERT2;Braf^CA/CA with Trp53^flox mice to generate Etv1^CreERT2;Braf^CA/+;Trp53^flox/flox and Etv1^CreERT2;Braf^CA/CA;Trp53^flox/flox compound genetically engineered mice.

Ran L., Murphy D., Sher J., Cao Z., Wang S., Walczak E., Guan Y., Xie Y., Shukla S., Zhan Y., Antonescu C.R., Chen Y, & Chi P. (2017). ETV1-positive cells give rise to BRAFV600E mutant gastrointestinal stromal tumors. Cancer research, 77(14), 3758-3765.

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